EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.21 | expressed in Pichia pastoris strain Y11430 | Aspergillus niger |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.21 | D463A | the variant exhibits kinetic constants similar to the wild type enzyme | Aspergillus niger |
3.2.1.21 | D487A | the variant exhibits kinetic constants similar to the wild type enzyme | Aspergillus niger |
3.2.1.21 | D496A | the variant exhibits kinetic constants similar to the wild type enzyme | Aspergillus niger |
3.2.1.21 | D501A | the variant exhibits kinetic constants similar to the wild type enzyme | Aspergillus niger |
3.2.1.21 | E490A | the variant exhibits greatly reduced kcat and kcat/Km values toward the natural substrate cellobiose (67000 and 61000fold, respectively) | Aspergillus niger |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | Sodium azide | about 50% reduced activity at 500 mM | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.015 | - |
2,4-dinitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 6.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.1 | - |
2,4-dinitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.26 | - |
2,4-dinitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 6.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.44 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D496A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.49 | - |
2,4-dinitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.54 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.55 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D487A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.57 | - |
cellobiose | mutant enzyme D496A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.64 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D463A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.97 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D501A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 1 | - |
cellobiose | mutant enzyme D463A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 1.02 | - |
cellobiose | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 1.03 | - |
cellobiose | mutant enzyme D487A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 1.11 | - |
cellobiose | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 1.36 | - |
cellobiose | mutant enzyme D501A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 6.09 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | cellobiose + H2O | Aspergillus niger | - |
2 beta-D-glucose | - |
? | |
3.2.1.21 | cellobiose + H2O | Aspergillus niger ASKU28 | - |
2 beta-D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Aspergillus niger | - |
- |
- |
3.2.1.21 | Aspergillus niger ASKU28 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 2,4-dinitrophenyl beta-D-glucoside + H2O | - |
Aspergillus niger | ? | - |
? | |
3.2.1.21 | 2,4-dinitrophenyl beta-D-glucoside + H2O | - |
Aspergillus niger ASKU28 | ? | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Aspergillus niger ASKU28 | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Aspergillus niger | 2 beta-D-glucose | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Aspergillus niger ASKU28 | 2 beta-D-glucose | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.001 | - |
cellobiose | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.32 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.34 | - |
2,4-dinitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 6.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 2.12 | - |
2,4-dinitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 47.3 | - |
cellobiose | mutant enzyme D501A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 67.4 | - |
cellobiose | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 71 | - |
2,4-dinitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 6.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 78.3 | - |
cellobiose | mutant enzyme D496A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 78.4 | - |
cellobiose | mutant enzyme D463A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 87.7 | - |
cellobiose | mutant enzyme D487A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 104 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D501A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 141 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D496A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 141 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 160 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D463A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 163 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D487A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 422 | - |
2,4-dinitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 4 | - |
- |
Aspergillus niger |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.001 | - |
cellobiose | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 0.05 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 21.2 | - |
2,4-dinitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 22.6 | - |
2,4-dinitrophenyl beta-D-glucoside | mutant enzyme E490A, in 0.1 M sodium acetate, pH 6.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 34.7 | - |
cellobiose | mutant enzyme D501A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 60.6 | - |
cellobiose | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 78.7 | - |
cellobiose | mutant enzyme D463A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 85.2 | - |
cellobiose | mutant enzyme D487A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 107 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D501A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 138 | - |
cellobiose | mutant enzyme D496A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 249 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D463A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 263 | - |
4-nitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 273 | - |
2,4-dinitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 6.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 297 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D487A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 321 | - |
4-nitrophenyl beta-D-glucoside | mutant enzyme D496A, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger | |
3.2.1.21 | 863 | - |
2,4-dinitrophenyl beta-D-glucoside | wild type enzyme, in 0.1 M sodium acetate, pH 4.0, at 40°C | Aspergillus niger |