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Literature summary extracted from

  • Yeung, J.H.; Telford, J.C.; Shidmoossavee, F.S.; Bennet, A.J.; Taylor, G.L.; Moore, M.M.
    Kinetic and structural evaluation of selected active site mutants of the Aspergillus fumigatus KDNase (sialidase) (2013), Biochemistry, 52, 9177-9186.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 expressed in Escherichia coli BL21(DE3) cells Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.18 D84A the mutation significantly lowers the activity of the enzyme, but this mutant remains a retaining glycosidase Aspergillus fumigatus
3.2.1.18 R171L the mutant has higher activity on a N-acetylneuraminic acid-based substrate than wild type enzyme Aspergillus fumigatus
3.2.1.18 Y358H the mutation significantly lowers the activity of the enzyme, but this mutant remains a retaining glycosidase Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Aspergillus fumigatus Q4WQS0
-
-
3.2.1.18 Aspergillus fumigatus Af293 Q4WQS0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 Ni-NTA column chromatography and Sephacryl S-100H gel filtration Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O preferred substrate Aspergillus fumigatus ?
-
 ?
3.2.1.18 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid + H2O preferred substrate Aspergillus fumigatus Af293 ?
-
 ?
3.2.1.18 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.18 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 Aspergillus fumigatus Af293 ?
-
 ?
3.2.1.18 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O
-
 Aspergillus fumigatus 8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate
-
 ?
3.2.1.18 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside + H2O
-
 Aspergillus fumigatus Af293 8-fluoromethylumbelliferyl alpha-D-galactopyranoside + 3-deoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 KDNase
-
 Aspergillus fumigatus
3.2.1.18 sialidase
-
 Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.18 8
-
 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 200
-
 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 330
-
 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 1620
-
 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 8900
-
 8-fluoromethylumbelliferyl alpha-2-oxo-3-deoxy-D-glycero-D-galacto-nononylgalactopyranoside mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 11600
-
 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 14500
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 19000
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme D84A, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 26800
-
 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid mutant enzyme Y358H, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 47700
-
 4-methylumbelliferyl alpha-2-oxo-3-deoxynononic acid wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 53500
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid wild type enzyme, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus
3.2.1.18 74200
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme R71L, pH 5.2, 16 mM sodium tartrate, at 37°C Aspergillus fumigatus