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Literature summary extracted from

  • Phillips, R.S.; Wang, A.K.; Marchal, S.; Lange, R.
    Effects of pressure and osmolytes on the allosteric equilibria of Salmonella typhimurium tryptophan synthase (2012), Biochemistry, 51, 9354-9363.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.20 betaine 1 M betaine activates the enzyme 1.8fold in the absence of monovalent cations Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 polyethylene glycol 8000 5% (w/v) activates the enzyme 2.1fold in the absence of monovalent cations Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 sucrose 2 M sucrose activates the enzyme 3.3fold in the absence of monovalent cations Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 taurine 0.5 M taurine activates the enzyme 3fold in the absence of monovalent cations Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20 expressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

EC Number General Stability Organism
4.2.1.20 in the presence of monovalent cations, osmolytes have only minor effects on activity and allosteric equilibria, but 1 M betaine stabilizes the Trp synthase-L-Ser-indoline complex against apparent pressure-induced subunit dissociation Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.20 K+ activates at 0.1 M Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 Na+ activates at 0.1 M Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 NH4+ activates at 0.1 M Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 Trp synthase
-
Salmonella enterica subsp. enterica serovar Typhimurium