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Literature summary extracted from

  • Babashpour, S.; Aminzadeh, S.; Farrokhi, N.; Karkhane, A.; Haghbeen, K.
    Characterization of a chitinase (Chit62) from Serratia marcescens B4A and its efficacy as a bioshield against plant fungal pathogens (2012), Biochem. Genet., 50, 722-735.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information Michaelis-Menten kinetics, 3.30 mg/ml with colloidal chitin, pH 6.0, 50°C, recombinant enzyme Serratia marcescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular the enzyme contains a 25 amino acid signal peptide and is secreted Serratia marcescens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 62000
-
 x * 62000, recombinant His6-tagged enzyme, SDS-PAGE Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Serratia marcescens
-
 ?
-
 ?
3.2.1.14 chitin + H2O Serratia marcescens B4A
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Serratia marcescens E5FGC5
-
-
3.2.1.14 Serratia marcescens B4A E5FGC5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant His6-tagged enzyme 5fold from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and dialysis Serratia marcescens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.14 chitopentaose + H2O = chitobiose + chitotriose double displacement reaction mechanism, overview Serratia marcescens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Serratia marcescens ?
-
 ?
3.2.1.14 chitin + H2O
-
 Serratia marcescens B4A ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Serratia marcescens ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Serratia marcescens B4A ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 62000, recombinant His6-tagged enzyme, SDS-PAGE Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chit62
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 55
-
-
 Serratia marcescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 15 85 activity range, over 50% activity at 30-60°C Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 50
-
 purified enzyme, pH 6.0, completely stable up to for 20 min Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6
-
-
 Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 10 activity range, over 50% of maximal activity at pH 5.0-8.0 Serratia marcescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 4 9 purified enzyme, 50°C, 1 h, over 50% activity remaing within this range, completely stable at pH 6.0-8.0 Serratia marcescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Serratia marcescens sequence calculation
-
 6.35

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution phylogenetic analysis reveals the possibility of horizontal gene transfer between bacteria, viruses, and insects. The Chit62 enzyme is clearly grouped with other bacterial chitinases, but with a clear distinction from previously reported Serratia marcescens Serratia marcescens
3.2.1.14 physiological function the active enzyme shows biocontrol features against fungal phytopathogens Serratia marcescens