Literature summary extracted from

Dou, S.; Kong, X.D.; Ma, B.D.; Chen, Q.; Zhang, J.; Zhou, J.; Xu, J.H.
Crystal structures of Pseudomonas putida esterase reveal the functional role of residues 187 and 287 in substrate binding and chiral recognition (2014), Biochem. Biophys. Res. Commun., 446, 1145-1150.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.1.1	expressed in Escherichia coli BL21(DE3) cells	Pseudomonas putida

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.1.1.1	sitting drop vapor diffusion method, using 0.1 M HEPES/MOPS-Na, pH 7.5, 12.5% (w/v) PEG1000, 12.5% (w/v) PEG3350, 12.5% (v/v) 2-methyl-2,4-pentanediol, 0.03 M MgCl2, and 0.03 M CaCl2	Pseudomonas putida

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.1.1	D287A	the mutant shows about 81fold increase in activity and significant decrease in enantioselectivity towards rac-2-acetoxy-2-(2'-chlorophenyl) acetate compared to the wild type	Pseudomonas putida
3.1.1.1	W187H	the mutant shows about 100fold increase in activity towards rac-2-acetoxy-2-(2'-chlorophenyl) acetate compared to the wild type	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.1	Pseudomonas putida	-	-	-
3.1.1.1	Pseudomonas putida ECU1011	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.1.1	Ni-NTA column chromatography and Superdex 75 gel filtration	Pseudomonas putida

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.1.1	rac-2-acetoxy-2-(2'-chlorophenyl) acetate + H2O	-	Pseudomonas putida	?	-	?
3.1.1.1	rac-2-acetoxy-2-(2'-chlorophenyl) acetate + H2O	-	Pseudomonas putida ECU1011	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.1.1	esterase	-	Pseudomonas putida
3.1.1.1	PPE	-	Pseudomonas putida

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)