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Literature summary extracted from

  • Devi, A.S.; Ebihara, A.; Kuramitsu, S.; Yokoyama, S.; Kumarevel, T.; Ponnuraj, K.
    Crystal structure of type I 3-dehydroquinate dehydratase of Aquifex aeolicus suggests closing of active site flap is not essential for enzyme action (2013), Biochem. Biophys. Res. Commun., 432, 350-354.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.10 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Aquifex aeolicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.10 the enzyme is co-crystallized with the ligand TLA(C4H6O6) or L(+)-tartaric acid, sitting drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate and 0.1 M citrate, pH 5.6 Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.10 27000
-
 2 * 27000, SDS-PAGE Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.10 3-dehydroquinate Aquifex aeolicus
-
 3-dehydroshikimate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.10 Aquifex aeolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.10 SuperQ Toyopearl 650 column chromatography, Resource Q column chromatography, hydroxylapatite column chromatography, and Superdex 200 gel filtration Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.10 3-dehydroquinate
-
 Aquifex aeolicus 3-dehydroshikimate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.10 homodimer 2 * 27000, SDS-PAGE Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.10 3-dehydroquinase
-
 Aquifex aeolicus
4.2.1.10 3-dehydroquinate dehydratase
-
 Aquifex aeolicus
4.2.1.10 3-DHQase
-
 Aquifex aeolicus
4.2.1.10 type I 3-dehydroquinate dehydratase
-
 Aquifex aeolicus