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Literature summary extracted from
- Crystal structure of LeuD from Methanococcus jannaschii (2012), Biochem. Biophys. Res. Commun., 419, 160-164.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.33 | expressed in Escherichia coli BL21(DE3) cells | Methanocaldococcus jannaschii |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.33 | sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000 and 0.1 MTris-HCl (pH 7.9) | Methanocaldococcus jannaschii |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.33 | 18000 | - |
x * 46000 + x * 18000, SDS-PAGE | Methanocaldococcus jannaschii |
| 4.2.1.33 | 46000 | - |
x * 46000 + x * 18000, SDS-PAGE | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.33 | (2R,3S)-3-isopropylmalate | Methanocaldococcus jannaschii | - |
2-isopropylmaleate + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.33 | Methanocaldococcus jannaschii | Q58673 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.33 | nickel-affinity column chromatography and Superdex 200 gel filtration | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.33 | (2R,3S)-3-isopropylmalate | - |
Methanocaldococcus jannaschii | 2-isopropylmaleate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.33 | ? | x * 46000 + x * 18000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.33 | 3-isopropylmalate isomerase | - |
Methanocaldococcus jannaschii |
| 4.2.1.33 | IPM isomerase | - |
Methanocaldococcus jannaschii |
| 4.2.1.33 | isopropylmalate isomerase | - |
Methanocaldococcus jannaschii |
| 4.2.1.33 | leuC | large subunit | Methanocaldococcus jannaschii |
| 4.2.1.33 | LeuD | small subunit | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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