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Literature summary extracted from

  • Vester, J.; Glaring, M.; Stougaard, P.
    An exceptionally cold-adapted alpha-amylase from a metagenomic library of a cold and alkaline environment (2015), Appl. Microbiol. Biotechnol., 99, 717-727.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Escherichia coli uncultured organism

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Ba2+ 2 mM, 72% residual activity uncultured organism
3.2.1.1 Bio-tex liquid commercial detergent, 75% residual activity uncultured organism
3.2.1.1 Cu2+ 2 mM, no residual activity uncultured organism
3.2.1.1 EDTA 1 mM, almost complete loss of activity uncultured organism
3.2.1.1 Green Balance solid commercial detergent, when used with tap water, 51% residual activity uncultured organism
3.2.1.1 Mg2+ 2 mM, 58% residual activity uncultured organism
3.2.1.1 sodium dodecylsulfate 0.1%, almost complete loss of activity uncultured organism
3.2.1.1 Zn2+ 2 mM, no residual activity uncultured organism

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 starch Km value is 1.79 ml/ml at 5°C, pH 8.3 and ,328 mg/ml at 15°C, pH 8.3 uncultured organism
3.2.1.1 additional information
-
 amylopectin Km value is 1.9 ml/ml at 5°C, pH 8.3 and 2.5 mg/ml at 15°C, pH 8.3 uncultured organism

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ca2+ 2 mM, 127% of initial activity uncultured organism

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 uncultured organism A0A060DAC6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant enzyme uncultured organism

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O
-
 uncultured organism ?
-
 ?
3.2.1.1 amylose + H2O
-
 uncultured organism ?
-
 ?
3.2.1.1 maltoheptaose + 3 H2O
-
 uncultured organism 2 maltose + maltotriose additional product: maltotetraose ?
3.2.1.1 maltopentaose + H2O
-
 uncultured organism maltose + maltotriose
-
 ?
3.2.1.1 maltotetraose + H2O
-
 uncultured organism 2 maltose weak activity ?
3.2.1.1 additional information no substrates: granular starch or glycogen, maltose, maltotriose uncultured organism ?
-
 ?
3.2.1.1 starch + H2O
-
 uncultured organism maltose + ? and larger maltooligosaccharides ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 AmyI3C6
-
 uncultured organism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 10 15
-
 uncultured organism

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 1
-
 more than 70% of maximum activity uncultured organism

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 34
-
 or below, 60 min, no loss of activity uncultured organism
3.2.1.1 45
-
 20 min, complete loss of activity uncultured organism
3.2.1.1 55
-
 5 min, complete loss of activity uncultured organism

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 0.03
-
 starch 5°C, pH 8.3 uncultured organism
3.2.1.1 0.042
-
 amylopectin 5°C, pH 8.3 uncultured organism
3.2.1.1 0.15
-
 amylopectin 15°C, pH 8.3 uncultured organism
3.2.1.1 0.156
-
 starch 15°C, pH 8.3 uncultured organism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 8 9
-
 uncultured organism

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 6 10 20°C, stable for at least 14 h uncultured organism