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Literature summary extracted from

  • Chen, M.; Lei, X.; Chen, C.; Zhang, S.; Xie, J.; Wei, D.
    Cloning, overexpression, and characterization of a highly active endoinulinase gene from Aspergillus fumigatus Cl1 for production of inulo-oligosaccharides (2014), Appl. Biochem. Biotechnol., 175, 1153-1167.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.7 synthesis immobilization of endoinulinase results in higher stability than the free endoinulinase under various temperature levels. A residual activity of 81.2% can be still obtained after ten reaction cycles Aspergillus fumigatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.7 expression in Pichia pastoris Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.7 Ag+ 1 mM, no resiudal activity Aspergillus fumigatus
3.2.1.7 Cu2+ 1 mM, 18% resiudal activity Aspergillus fumigatus
3.2.1.7 EDTA 1 mM, 6.7% resiudal activity Aspergillus fumigatus
3.2.1.7 sodium dodecylsulfate 40 mM, no resiudal activity Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.7 2.18
-
 inulin pH 6.0, 55°C Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.7 55000
-
 x * 55000, SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.7 Aspergillus fumigatus
-
-
-
3.2.1.7 Aspergillus fumigatus Cl1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.7 recombinant protein Aspergillus fumigatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.7 1520
-
 pH 6.0, 55°C Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.7 inulin + H2O
-
 Aspergillus fumigatus ?
-
 ?
3.2.1.7 inulin + H2O
-
 Aspergillus fumigatus Cl1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.7 ? x * 55000, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.7 55
-
-
 Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.7 6
-
-
 Aspergillus fumigatus