EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.8 | synthesis | production of enzyme in Pichia pastoris with codon optimization. The activity of dual-copy enzyme is maximized at 15158 U/ml after 120 h of shaking | Aspergillus niger |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.8 | expression in Pichia pastoris | Aspergillus niger |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | Ba2+ | 10 mM, 77% residual activity | Aspergillus niger | |
3.2.1.8 | Fe2+ | 10 mM, 83% residual activity | Aspergillus niger | |
3.2.1.8 | sodium dodecylsulfate | 10 mM, 72% residual activity | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.8 | additional information | - |
xylan | Km value is 18.27 mg/ml, pH 5.0, 50°C | Aspergillus niger |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | Co2+ | 10 mM, 119% of initial activity | Aspergillus niger | |
3.2.1.8 | Tween 80 | 0.5%, 185% of initial activity | Aspergillus niger |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 23000 | - |
x * 23000, SDS-PAGE | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Aspergillus niger | F5CI28 | - |
- |
3.2.1.8 | Aspergillus niger IA-0010 | F5CI28 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6853 | - |
substrate birchwood xylan, pH 5.0, 50°C | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | additional information | almost no activity on xylobiose | Aspergillus niger | ? | - |
? | |
3.2.1.8 | additional information | almost no activity on xylobiose | Aspergillus niger IA-0010 | ? | - |
? | |
3.2.1.8 | xylan + H2O | substrate birchwood xylan | Aspergillus niger | xylobiose + D-xylose + ? | after 24 h of hydrolysis, more than 90% of the total hydrolysis products of xylan are xylobiose and D-xylose with almost no xylotetraose, -pentaose, or -hexaose | ? | |
3.2.1.8 | xylan + H2O | substrate birchwood xylan | Aspergillus niger IA-0010 | xylobiose + D-xylose + ? | after 24 h of hydrolysis, more than 90% of the total hydrolysis products of xylan are xylobiose and D-xylose with almost no xylotetraose, -pentaose, or -hexaose | ? | |
3.2.1.8 | xylohexaose + H2O | - |
Aspergillus niger | xylobiose + D-xylose + ? | - |
? | |
3.2.1.8 | xylohexaose + H2O | - |
Aspergillus niger IA-0010 | xylobiose + D-xylose + ? | - |
? | |
3.2.1.8 | xylopentaose + H2O | - |
Aspergillus niger | xylobiose + D-xylose | - |
? | |
3.2.1.8 | xylotetraose + H2O | - |
Aspergillus niger | 2 xylobiose | - |
? | |
3.2.1.8 | xylotetraose + H2O | - |
Aspergillus niger IA-0010 | 2 xylobiose | - |
? | |
3.2.1.8 | xylotriose + H2O | - |
Aspergillus niger | xylobiose + D-xylose | - |
? | |
3.2.1.8 | xylotriose + H2O | - |
Aspergillus niger IA-0010 | xylobiose + D-xylose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.8 | ? | x * 23000, SDS-PAGE | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | XynB | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 50 | - |
- |
Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 70 | - |
10 min, complete loss of activity | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 5 | - |
- |
Aspergillus niger |