Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hegde, K.; Veeranki, V.D.
    Production optimization and characterization of recombinant cutinases from Thermobifida fusca sp. NRRL B-8184 (2013), Appl. Biochem. Biotechnol., 170, 654-675.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.74 DMF activates 10% at 40% v/v Thermobifida fusca
3.1.1.74 DMF activates 6% at 40% v/v Thermobifida fusca
3.1.1.74 DMSO activates 14% at 40% v/v Thermobifida fusca
3.1.1.74 DMSO activates 9% at 40% v/v Thermobifida fusca
3.1.1.74 sodium 2-{[(3alpha,5beta,12alpha)-3,12-dihydroxy-24-oxocholan-24-yl]amino}ethane-1-sulfonate activates 10% at 10 mM Thermobifida fusca
3.1.1.74 sodium 2-{[(3alpha,5beta,12alpha)-3,12-dihydroxy-24-oxocholan-24-yl]amino}ethane-1-sulfonate activates 24% at 10 mM Thermobifida fusca

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.74 gene cut1, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), secretion into periplasm and medium, method development and optimization for process for large-scale production of cutinase in soluble form Thermobifida fusca
3.1.1.74 gene cut2, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), secretion into periplasm and medium, method development and optimization for process for large-scale production of cutinase in soluble form Thermobifida fusca

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.74 1-Heptanol 27% inhibition at 40% v/v; 28% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 1-Hexanol 68% inhibition at 40% v/v; 86% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 AgNO3 90% inhibition at 1 mM; 93% inhibition at 1 mM Thermobifida fusca
3.1.1.74 BaCl2 14% inhibition at 1 mM; 8% inhibition at 1 mM Thermobifida fusca
3.1.1.74 Benzene 77% inhibition at 40% v/v; 79% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 butanol 10% inhibition at 40% v/v; 17% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 CrCl2 99% inhibition at 1 mM; 99% inhibition at 1 mM Thermobifida fusca
3.1.1.74 CuSO4 26% inhibition at 1 mM; 32% inhibition at 1 mM Thermobifida fusca
3.1.1.74 deoxycholate 24% inhibition at 10 mM; 25% inhibition at 10 mM Thermobifida fusca
3.1.1.74 Dichloromethane 61% inhibition at 40% v/v; 74% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 diethyldicarbonate 54% inhibition at 1 mM; 56% inhibition at 1 mM Thermobifida fusca
3.1.1.74 FeSO4 55% inhibition at 1 mM; 55% inhibition at 1 mM Thermobifida fusca
3.1.1.74 hexyl acetate 63% inhibition at 40% v/v; 83% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 HgCl2 99% inhibition at 1 mM; 99% inhibition at 1 mM Thermobifida fusca
3.1.1.74 methanol 8% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 MgCl2 15% inhibition at 1 mM; 8% inhibition at 1 mM Thermobifida fusca
3.1.1.74 additional information no or poor inhibition by tetrahydrofuran, n-hexane, methanol, ethanol, acetone, and acetonitrile at 40% v/v, or by 10 mM sodium deoxycholate and 1 mM EDTA; no or poor inhibition by tetrahydrofuran, triethylamine, ethanol, acetone, and acetonitrile at 40% v/v, or by 10 mM sodium deoxycholate and 1 mM EDTA Thermobifida fusca
3.1.1.74 n-hexane 10% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 PbCl2 53% inhibition at 1 mM; 54% inhibition at 1 mM Thermobifida fusca
3.1.1.74 PMSF 48% inhibition at 1 mM; 59% inhibition at 1 mM Thermobifida fusca
3.1.1.74 RbCl 93% inhibition at 1 mM; 95% inhibition at 1 mM Thermobifida fusca
3.1.1.74 SDS 30% inhibition at 1 mM; 9% inhibition at 1 mM Thermobifida fusca
3.1.1.74 triethylamine 24% inhibition at 40% v/v Thermobifida fusca
3.1.1.74 Triton X-100 35% inhibition at 1 mM; 55% inhibition at 1 mM Thermobifida fusca
3.1.1.74 Tween-20 28% inhibition at 1 mM; 35% inhibition at 1 mM Thermobifida fusca
3.1.1.74 Tween-80 24% inhibition at 1 mM; 60% inhibition at 1 mM Thermobifida fusca
3.1.1.74 Urea 17% inhibition at 1 mM; 30% inhibition at 1 mM Thermobifida fusca
3.1.1.74 ZnSO4 59% inhibition at 1 mM; 65% inhibition at 1 mM Thermobifida fusca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.74 additional information
-
 additional information Michaelis-Menten kinetics Thermobifida fusca

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.74 KCl activates 12% at 1 mM Thermobifida fusca
3.1.1.74 additional information no effect on enzyme activity by 1 mM CoCl2, KCl, NiCl2, MgSO4, and MnCl2 Thermobifida fusca
3.1.1.74 additional information no effect on enzyme activity by 1 mM CoCl2, MnCl2, and MgSO4 Thermobifida fusca
3.1.1.74 NaCl activates 15% at 1 mM Thermobifida fusca
3.1.1.74 NaCl activates 20% at 1 mM Thermobifida fusca
3.1.1.74 NiCl2 activates 11% at 1 mM Thermobifida fusca

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.74 cutin + H2O Thermobifida fusca
-
 cutin monomers
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Thermobifida fusca G8GER6 gene Cut1
-
3.1.1.74 Thermobifida fusca Q6A0I4 gene cut2
-
3.1.1.74 Thermobifida fusca NRRL B-8184 G8GER6 gene Cut1
-
3.1.1.74 Thermobifida fusca NRRL B-8184 Q6A0I4 gene cut2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.74 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) culture supernatant by nickel affinity chromatography to homogeneity Thermobifida fusca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.74 0.218
-
 pH 8.0, 50°C, purified recombinant His-tagged enzyme, 4-nitrophenyl (16-methyl sulfoyl ester) hexadecanoate Thermobifida fusca
3.1.1.74 0.395
-
 pH 8.0, 50°C, purified recombinant His-tagged enzyme, 4-nitrophenyl (16-methyl sulfone ester) hexadecanoate Thermobifida fusca
3.1.1.74 542.5
-
 pH 8.0, 50°C, purified recombinant His-tagged enzyme, substrate 4-nitrophenyl butyrate Thermobifida fusca
3.1.1.74 643.4
-
 pH 8.0, 50°C, purified recombinant His-tagged enzyme, substrate 4-nitrophenyl butyrate Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl (16-methyl sulfone ester) hexadecanoate + H2O
-
 Thermobifida fusca ?
-
 ?
3.1.1.74 4-nitrophenyl (16-methyl sulfonyl ester) hexadecanoate + H2O
-
 Thermobifida fusca 4-nitrophenol + (16-methyl sulfonyl ester) hexadecanoate
-
 ?
3.1.1.74 4-nitrophenyl acetate + H2O high activity Thermobifida fusca 4-nitrophenol + acetate
-
 ?
3.1.1.74 4-nitrophenyl acetate + H2O high activity Thermobifida fusca NRRL B-8184 4-nitrophenol + acetate
-
 ?
3.1.1.74 4-nitrophenyl butyrate + H2O best 4-nitrophenyl ester substrate Thermobifida fusca 4-nitrophenol + butyrate
-
 ?
3.1.1.74 4-nitrophenyl butyrate + H2O best 4-nitrophenyl ester substrate Thermobifida fusca NRRL B-8184 4-nitrophenol + butyrate
-
 ?
3.1.1.74 4-nitrophenyl decanoate + H2O
-
 Thermobifida fusca 4-nitrophenol + decanoate
-
 ?
3.1.1.74 4-nitrophenyl decanoate + H2O
-
 Thermobifida fusca NRRL B-8184 4-nitrophenol + decanoate
-
 ?
3.1.1.74 4-nitrophenyl laurate + H2O
-
 Thermobifida fusca 4-nitrophenol + laurate
-
 ?
3.1.1.74 4-nitrophenyl laurate + H2O low activity Thermobifida fusca 4-nitrophenol + laurate
-
 ?
3.1.1.74 4-nitrophenyl myristate + H2O low activity Thermobifida fusca 4-nitrophenol + myristate
-
 ?
3.1.1.74 4-nitrophenyl octanoate + H2O
-
 Thermobifida fusca 4-nitrophenol + octanoate
-
 ?
3.1.1.74 4-nitrophenyl octanoate + H2O
-
 Thermobifida fusca NRRL B-8184 4-nitrophenol + octanoate
-
 ?
3.1.1.74 4-nitrophenyl palmitate + H2O low activity Thermobifida fusca 4-nitrophenol + palmitate
-
 ?
3.1.1.74 4-nitrophenyl valerate + H2O
-
 Thermobifida fusca 4-nitrophenol + valerate
-
 ?
3.1.1.74 4-nitrophenyl valerate + H2O
-
 Thermobifida fusca NRRL B-8184 4-nitrophenol + valerate
-
 ?
3.1.1.74 cutin + H2O
-
 Thermobifida fusca cutin monomers
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.74 Cut1
-
 Thermobifida fusca
3.1.1.74 Cut2
-
 Thermobifida fusca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.74 55
-
-
 Thermobifida fusca

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.74 40 80 activity range Thermobifida fusca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.74 37
-
 purified recombinant enzyme, residual activity is over 75% after 125 h Thermobifida fusca
3.1.1.74 55
-
 purified recombinant enzyme, residual activity is over 38% after 125 h Thermobifida fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 8
-
-
 Thermobifida fusca

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.74 6.8 9 activity range Thermobifida fusca

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.74 1350
-
 4-nitrophenyl butyrate pH 8.0, 50°C Thermobifida fusca
3.1.1.74 2840
-
 4-nitrophenyl butyrate pH 8.0, 50°C Thermobifida fusca