EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.8 | expression in Escherichia coli | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.36 | - |
myo-inositol hexakisphosphate | pH 7.4, 37°C, presence of Ca2+ | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Ca2+ | required, assay in presence of 1.5 mM. The removal of Ca2+ causes a change in the CD-spectrum. The addition of Ca2+ to the apoenzyme results in an increase in the mean residue ellipticity, indicating a recovery of beta-sheet elements | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 47500 | - |
x * 47500, SDS-PAGE | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Bacillus subtilis | H9C9P0 | subsp. spizizenii | - |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.1.3.8 | metal-depleted apoenzyme can be restored by Ca2+, Sr2+,Co2+, Ba2+, Cu2+, La3+, Ni+, Tb2+, and Mn2+ to 44.1%, 29.8%, 36.7%, 26.9%, 1.9%, 30.0%, 34.2%, 8.3%, and 34.9% of its reference activity, respectively | Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 14 | - |
pH 7.4, 37°C, presence of Ca2+ | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.8 | ? | x * 47500, SDS-PAGE | Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 50 | - |
- |
Bacillus subtilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 47.8 | - |
melting temperature | Bacillus subtilis |
3.1.3.8 | 62.4 | - |
melting temperature, presence of Ca2+ | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 25.4 | - |
myo-inositol hexakisphosphate | pH 7.4, 37°C, presence of Ca2+ | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 6 | - |
- |
Bacillus subtilis |