Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ullah, A.; Magalhaes, G.S.; Masood, R.; Mariutti, R.B.; Coronado, M.A.; Murakami, M.T.; Barbaro, K.C.; Arni, R.K.
    Crystallization and preliminary X-ray diffraction analysis of a novel sphingomyelinase D from Loxosceles gaucho venom (2014), Acta Crystallogr. Sect. F, 70, 1418-1420.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.41 recombinant expression in Escherichia coli strain BL21 Star(DE3)pLysS Loxosceles gaucho

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.41 purified recombinant enzyme, hanging drop vapour diffusion method, mixxing of 0.001 ml of 15 mg/ml protein in phosphate buffer, pH 7.0, with 0.001 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.2, 22% PEG 10000, equilibration against 0.5 ml reservoir solution, 18°C, X-ray diffraction structure determination and anaysis at 2.6 A resolution Loxosceles gaucho

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.41 extracellular
-
 Loxosceles gaucho
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.41 2-lysophosphatidylcholine + H2O Loxosceles gaucho
-
 lysophosphatidic acid + choline
-
 ?
3.1.4.41 sphingomyelin + H2O Loxosceles gaucho
-
 ceramide 1-phosphate + choline
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.41 Loxosceles gaucho K9USW8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.41 recombinant enzme from Escherichia coli strain BL21 Star(DE3)pLysS by affinity chromatography Loxosceles gaucho

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.41 venom
-
 Loxosceles gaucho
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.41 2-lysophosphatidylcholine + H2O
-
 Loxosceles gaucho lysophosphatidic acid + choline
-
 ?
3.1.4.41 sphingomyelin + H2O
-
 Loxosceles gaucho ceramide 1-phosphate + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.41 LgRec1
-
 Loxosceles gaucho
3.1.4.41 SMase D
-
 Loxosceles gaucho
3.1.4.41 sphingomyelinase D
-
 Loxosceles gaucho