Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nagai, K.; Yasuhira, K.; Tanaka, Y.; Kato, D.; Takeo, M.; Higuchi, Y.; Negoro, S.; Shibata, N.
    Crystallization and X-ray diffraction analysis of nylon hydrolase (NylC) from Arthrobacter sp. KI72 (2013), Acta Crystallogr. Sect. F, 69, 1151-1154.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.117 expressed in Escherichia coli JM109 cells Arthrobacter sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.117 sitting drop vapor diffusion method, with ammonium sulfate as a precipitant in 0.1 M HEPES buffer pH 7.5 containing 0.2 M NaCl and 25% (v/v) glycerol Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.117 9000
-
 1 * 27000 + 1 * 9000, SDS-PAGE Arthrobacter sp.
3.5.1.117 27000
-
 1 * 27000 + 1 * 9000, SDS-PAGE Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.117 [N-(6-aminohexanoyl)]n + H2O Arthrobacter sp.
-
 [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x
-
 ?
3.5.1.117 [N-(6-aminohexanoyl)]n + H2O Arthrobacter sp. KI72
-
 [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.117 Arthrobacter sp.
-
-
-
3.5.1.117 Arthrobacter sp. KI72
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.117 ammonium sulfate precipitation, Q Sepharose column chromatography, Sephacryl S-200 and gel filtration Arthrobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.117 [N-(6-aminohexanoyl)]n + H2O
-
 Arthrobacter sp. [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x
-
 ?
3.5.1.117 [N-(6-aminohexanoyl)]n + H2O
-
 Arthrobacter sp. KI72 [N-(6-aminohexanoyl)]n-x + [N-(6-aminohexanoyl)]x
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.117 heterodimer 1 * 27000 + 1 * 9000, SDS-PAGE Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.1.117 NylC
-
 Arthrobacter sp.