Literature summary extracted from

Pendini, N.R.; Yap, M.Y.; Traore, D.A.; Polyak, S.W.; Cowieson, N.P.; Abell, A.; Booker, G.W.; Wallace, J.C.; Wilce, J.A.; Wilce, M.C.
Structural characterization of Staphylococcus aureus biotin protein ligase and interaction partners: an antibiotic target (2013), Protein Sci., 22, 762-773.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.3.4.15	expressed in Escherichia coli BL21 cells	Staphylococcus aureus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
6.3.4.15	apoenzyme, in complex with biotin, and complexed with biotinyl-5'-AMP, hanging drop vapor diffusion method, using	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.3.4.15	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Staphylococcus aureus	-	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
6.3.4.15	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Staphylococcus aureus ECT-R2	-	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.3.4.15	Staphylococcus aureus	-	-	-
6.3.4.15	Staphylococcus aureus ECT-R2	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.3.4.15	-	Staphylococcus aureus

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.3.4.15	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	Staphylococcus aureus	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
6.3.4.15	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	Staphylococcus aureus ECT-R2	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
6.3.4.15	additional information	biotin protein ligase is a bifunctional protein, possessing both biotin ligase and transcription repressor activities	Staphylococcus aureus	?	-	?
6.3.4.15	additional information	biotin protein ligase is a bifunctional protein, possessing both biotin ligase and transcription repressor activities	Staphylococcus aureus ECT-R2	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.3.4.15	biotin protein ligase	-	Staphylococcus aureus
6.3.4.15	BPL	-	Staphylococcus aureus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
6.3.4.15	ATP	-	Staphylococcus aureus

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Release 2023.1 (January 2023)