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Literature summary extracted from

  • Liebold, C.; List, F.; Kalbitzer, H.R.; Sterner, R.; Brunner, E.
    The interaction of ammonia and xenon with the imidazole glycerol phosphate synthase from Thermotoga maritima as detected by NMR spectroscopy (2010), Protein Sci., 19, 1774-1782.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.B2 expression in Escherichia coli Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.B2 T78M the mutation does not impair substrate binding to the active site of HisF Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.B2 0.002
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: L-glutamine, wild-type enzyme Thermotoga maritima
4.3.1.B2 0.0028
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: L-glutamine, HisH/HisF holoenzyme with a T78M mutant subunit HisF Thermotoga maritima
4.3.1.B2 0.0036
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: NH4+, wild-type enzyme Thermotoga maritima
4.3.1.B2 0.0059
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: NH4+, HisH/HisF holoenzyme with a T78M mutant subunit HisF Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.B2 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.B2
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.B2 L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Thermotoga maritima L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
4.3.1.B2 NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Thermotoga maritima D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.B2 25
-
 assay at Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.B2 1.1
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: L-glutamine, wild-type enzyme Thermotoga maritima
4.3.1.B2 1.2
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: NH4+, wild-type enzyme Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.B2 8
-
 assay at Thermotoga maritima