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Literature summary extracted from
- Cloning, purification, and characterization of inorganic pyrophosphatase from the hyperthermophilic archaea Pyrococcus horikoshii (2014), Protein Expr. Purif., 99, 94-98.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.6.1.1 | expression in Escherichia coli | Pyrococcus horikoshii |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.1 | 0.0143 | - |
diphosphate | pH 8.0, 90°C | Pyrococcus horikoshii |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.6.1.1 | Mg2+ | maximum activity in the presence of Mg2+, influence of other metal cations is negligible | Pyrococcus horikoshii | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.6.1.1 | urea | the enzyme retains 40.66% of the original activity in the presence of 8 M urea after incubation for 24 h at 50°C and 39.3% of the original activity in the presence of 6 M urea at 50°C after incubation for 24 h. It retains 25.96% of the original activity in the presence of 8 M urea after incubation at 50°C for 120 h | Pyrococcus horikoshii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.6.1.1 | Pyrococcus horikoshii | O59570 | - |
- |
| 3.6.1.1 | Pyrococcus horikoshii DSM 12428 | O59570 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.6.1.1 | - |
Pyrococcus horikoshii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 101.6 | - |
pH 8.0, 90°C | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.6.1.1 | diphosphate + H2O | the enzyme shows high specificity for diphosphate but low reactivity to sodium tripolyphosphate and sodium tetrapolyphosphate. ADP and ATP can not serve as substrates | Pyrococcus horikoshii | 2 phosphate | - |
? | |
| 3.6.1.1 | diphosphate + H2O | the enzyme shows high specificity for diphosphate but low reactivity to sodium tripolyphosphate and sodium tetrapolyphosphate. ADP and ATP can not serve as substrates | Pyrococcus horikoshii DSM 12428 | 2 phosphate | - |
? | |
| 3.6.1.1 | tetrapolyphosphate + H2O | 25.2% activity compared to the activity with diphosphate | Pyrococcus horikoshii | ? | - |
? | |
| 3.6.1.1 | tetrapolyphosphate + H2O | 25.2% activity compared to the activity with diphosphate | Pyrococcus horikoshii DSM 12428 | ? | - |
? | |
| 3.6.1.1 | tripolyphosphate + H2O | 40.3% activity compared to the activity with diphosphate | Pyrococcus horikoshii | ? | - |
? | |
| 3.6.1.1 | tripolyphosphate + H2O | 40.3% activity compared to the activity with diphosphate | Pyrococcus horikoshii DSM 12428 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.6.1.1 | PH1907 | - |
Pyrococcus horikoshii |
| 3.6.1.1 | PPIase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 88 | - |
- |
Pyrococcus horikoshii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 70 | 100 | 70°C: about 50% of maximal activity, 100°C: about 50% of maximal activity | Pyrococcus horikoshii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 25 | - |
the enzyme remains active for 7 days | Pyrococcus horikoshii |
| 3.6.1.1 | 95 | - |
half-life: 5 h | Pyrococcus horikoshii |
| 3.6.1.1 | 100 | - |
half-life: 4 h, the enzyme retains 19.61% of the original activity after incubation at 100°C for 12 h | Pyrococcus horikoshii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.6.1.1 | 3436 | - |
diphosphate | pH 8.0, 90°C | Pyrococcus horikoshii | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 10.3 | - |
- |
Pyrococcus horikoshii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.6.1.1 | 8.5 | 11.5 | pH 8.5: about 50% of maximal activity, pH 11.5: about 55% of maximal activity | Pyrococcus horikoshii |
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