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Literature summary extracted from
- Recombinant expression, purification and characterisation of the native glutamate racemase from Lactobacillus plantarum NC8 (2013), Protein Expr. Purif., 88, 54-60.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.3 | gene murI from strain NC-8, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)/pET20b-murI | Lactiplantibacillus plantarum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.3 | 0.84 | - |
L-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum |  |
| 5.1.1.3 | 1.64 | - |
D-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 30000 | - |
2 * 30000, recombinant enzyme, SDS-PAGE | Lactiplantibacillus plantarum |
| 5.1.1.3 | 55800 | - |
recombinant enzyme, gel filtration | Lactiplantibacillus plantarum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.3 | L-glutamate | Lactiplantibacillus plantarum | - |
D-glutamate | - |
r | |
| 5.1.1.3 | L-glutamate | Lactiplantibacillus plantarum NC8 | - |
D-glutamate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.3 | Lactiplantibacillus plantarum | D7VBA0 | gene murI | - |
| 5.1.1.3 | Lactiplantibacillus plantarum NC8 | D7VBA0 | gene murI | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.3 | recombinant enzyme 9.16fold from Escherichia coli strain BL21(DE3)/pET20b-murI by L-Glu affinity and anion exchange chromatography | Lactiplantibacillus plantarum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 2043 | - |
purified recombinant enzyme, pH 7.5, 37°C | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.3 | D-glutamate | - |
Lactiplantibacillus plantarum | L-glutamate | - |
r | |
| 5.1.1.3 | D-glutamate | - |
Lactiplantibacillus plantarum NC8 | L-glutamate | - |
r | |
| 5.1.1.3 | L-glutamate | - |
Lactiplantibacillus plantarum | D-glutamate | - |
r | |
| 5.1.1.3 | L-glutamate | - |
Lactiplantibacillus plantarum NC8 | D-glutamate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.1.3 | dimer | 2 * 30000, recombinant enzyme, SDS-PAGE | Lactiplantibacillus plantarum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.1.3 | glutamic acid racemases | - |
Lactiplantibacillus plantarum |
| 5.1.1.3 | MurI | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 50 | - |
- |
Lactiplantibacillus plantarum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 20 | 50 | activity range, optimum at 50°C, inactive at 55°C | Lactiplantibacillus plantarum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.3 | 3029 | - |
L-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum | |
| 5.1.1.3 | 5980 | - |
D-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 7.5 | - |
assay at | Lactiplantibacillus plantarum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 7 | 10 | activity range | Lactiplantibacillus plantarum |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.3 | 7 | 10 | 97% activity remaining after 22 h at pH 7.0-8.0, half-life, by extrapolation of the data, is 287 h at pH 7.0, 154 h at pH 8.0, 25 h at pH 9.0, and 6 h at pH 10.0 | Lactiplantibacillus plantarum |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.1.3 | additional information | MurI from Lactobacillus plantarum NC8 seems to exhibit pseudosymmetry for the racemisation of Glu in both directions | Lactiplantibacillus plantarum |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.3 | 3606 | - |
L-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum | |
| 5.1.1.3 | 3646 | - |
D-glutamate | pH 7.5, 37°C | Lactiplantibacillus plantarum | |
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