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Literature summary extracted from

  • Yang, Z.Y.; Khadka, N.; Lukoyanov, D.; Hoffman, B.M.; Dean, D.R.; Seefeldt, L.C.
    On reversible H2 loss upon N2 binding to FeMo-cofactor of nitrogenase (2013), Proc. Natl. Acad. Sci. USA, 110, 16327-16332.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 additional information Azotobacter vinelandii in the draft mechanism model, H2 is produced by reductive elimination of the two bridging hydrides of a four-electron reduced intermediate during N2 binding. This process releases H2, yielding N2 bound to FeMo-cofactor that is doubly reduced relative to the resting redox level, and thereby is activated to promptly generate bound diazene. This mechanism predicts that during turnover under D2/N2, the reverse reaction of D2 with the N2-bound product of reductive elimination would generate a dideutero-four-electron reduced intermediate, which can relax with loss of HD to the state designated two-electron reduced intermediate, with a single deuteride bridge. The predicted two-electron reduced intermediate(D) and four-electron reduced intermediate(2D) states are established by intercepting them with the nonphysiological substrate acetylene to generate deuterated ethylenes. That gaseous H2/D2 can reduce a substrate other than H+ with N2 as a cocatalyst confirms the essential mechanistic role for H2 formation, and hence a limiting stoichiometry for biological nitrogen fixation of eight electrons/protons ?
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 ?
1.18.6.1 additional information Azotobacter vinelandii DJ1260 in the draft mechanism model, H2 is produced by reductive elimination of the two bridging hydrides of a four-electron reduced intermediate during N2 binding. This process releases H2, yielding N2 bound to FeMo-cofactor that is doubly reduced relative to the resting redox level, and thereby is activated to promptly generate bound diazene. This mechanism predicts that during turnover under D2/N2, the reverse reaction of D2 with the N2-bound product of reductive elimination would generate a dideutero-four-electron reduced intermediate, which can relax with loss of HD to the state designated two-electron reduced intermediate, with a single deuteride bridge. The predicted two-electron reduced intermediate(D) and four-electron reduced intermediate(2D) states are established by intercepting them with the nonphysiological substrate acetylene to generate deuterated ethylenes. That gaseous H2/D2 can reduce a substrate other than H+ with N2 as a cocatalyst confirms the essential mechanistic role for H2 formation, and hence a limiting stoichiometry for biological nitrogen fixation of eight electrons/protons ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Azotobacter vinelandii
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1.18.6.1 Azotobacter vinelandii DJ1260
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 additional information in the draft mechanism model, H2 is produced by reductive elimination of the two bridging hydrides of a four-electron reduced intermediate during N2 binding. This process releases H2, yielding N2 bound to FeMo-cofactor that is doubly reduced relative to the resting redox level, and thereby is activated to promptly generate bound diazene. This mechanism predicts that during turnover under D2/N2, the reverse reaction of D2 with the N2-bound product of reductive elimination would generate a dideutero-four-electron reduced intermediate, which can relax with loss of HD to the state designated two-electron reduced intermediate, with a single deuteride bridge. The predicted two-electron reduced intermediate(D) and four-electron reduced intermediate(2D) states are established by intercepting them with the nonphysiological substrate acetylene to generate deuterated ethylenes. That gaseous H2/D2 can reduce a substrate other than H+ with N2 as a cocatalyst confirms the essential mechanistic role for H2 formation, and hence a limiting stoichiometry for biological nitrogen fixation of eight electrons/protons Azotobacter vinelandii ?
-
 ?
1.18.6.1 additional information in the draft mechanism model, H2 is produced by reductive elimination of the two bridging hydrides of a four-electron reduced intermediate during N2 binding. This process releases H2, yielding N2 bound to FeMo-cofactor that is doubly reduced relative to the resting redox level, and thereby is activated to promptly generate bound diazene. This mechanism predicts that during turnover under D2/N2, the reverse reaction of D2 with the N2-bound product of reductive elimination would generate a dideutero-four-electron reduced intermediate, which can relax with loss of HD to the state designated two-electron reduced intermediate, with a single deuteride bridge. The predicted two-electron reduced intermediate(D) and four-electron reduced intermediate(2D) states are established by intercepting them with the nonphysiological substrate acetylene to generate deuterated ethylenes. That gaseous H2/D2 can reduce a substrate other than H+ with N2 as a cocatalyst confirms the essential mechanistic role for H2 formation, and hence a limiting stoichiometry for biological nitrogen fixation of eight electrons/protons Azotobacter vinelandii DJ1260 ?
-
 ?