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Literature summary extracted from

  • Tanwar, A.S.; Goyal, V.D.; Choudhary, D.; Panjikar, S.; Anand, R.
    Importance of hydrophobic cavities in allosteric regulation of formylglycinamide synthetase: insight from xenon trapping and statistical coupling analysis (2013), PLoS ONE, 8, e77781.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.3 expressed in Escherichia coli BL21DE3-834 (gold) cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.5.3 mutants R1263A and F209W, vapor diffusion method Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.3 F209W both the secondary structure content and activity of the mutant enzyme is very similar to the wild type protein Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 L1181F the mutation causes a 60% loss in activity Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 L1181W the mutant has secondary structure content and enzyme at par with the wild type protein and exhibits almost no destabilization Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 L1181Y the mutation results in failure to yield soluble protein Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 R1263A the mutant is structurally identical to the the native protein Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 T683W the mutation causes a 70% loss in activity Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.3 140000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.3 ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.3 Salmonella enterica subsp. enterica serovar Typhimurium P74881
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.3 Ni-NTA resin column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.3 ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.3 FGAM synthetase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 FGAR-AT
-
 Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 formylglycinamide ribonucleotide amidotransferase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 formylglycinamide synthetase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
6.3.5.3 PurL
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.5.3 42 80 the first unfolding transition at 42°C leads to a loss of 15-20% of the secondary structure content of the protein and 80% loss in activity. The second unfolding transition at 80°C represents unfolding of the bulk of the protein Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.3 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium