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Literature summary extracted from

  • Niehaus, T.D.; Richardson, L.G.; Gidda, S.K.; Elbadawi-Sidhu, M.; Meissen, J.K.; Mullen, R.T.; Fiehn, O.; Hanson, A.D.
    Plants utilize a highly conserved system for repair of NADH and NADPH hydrates (2014), Plant Physiol., 165, 52-61.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.93 recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts Arabidopsis thaliana
4.2.1.93 recombinant expression as GFP-tagged enzyme in Nicotiana tabacum suspension cells, subcellular localization comprises cytosol, mitochondria, and chloroplasts Zea mays
4.2.1.136 transient expression of GFP-tagged enzyme in Nicotiana tabacum BY-2 cells, expression of N-terminally His-tagged mature dehydratase in Escherichia coli strain BL21 (DE3) RIPL Arabidopsis thaliana
5.1.99.6 expression of N-terminally His-tagged enzyme in Escherichia coli Zea mays
5.1.99.6 expression of N-terminally His-tagged enzyme in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.136 additional information construction of enzyme knockout lines using T-DNA insertion, phenotype, overview Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.93 0.00069
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
4.2.1.93 0.00086
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
4.2.1.93 0.00089
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays
4.2.1.93 0.00115
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana
4.2.1.136 0.00069
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
4.2.1.136 0.00086
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
4.2.1.136 0.00089
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays
4.2.1.136 0.00115
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana
5.1.99.6 0.0006
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH and temperature not specified in the publication Arabidopsis thaliana
5.1.99.6 0.00115
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH and temperature not specified in the publication Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.93 chloroplast import assays with purified pea (Pisum sativum) chloroplasts and mitochondria Arabidopsis thaliana 9507
-
4.2.1.93 chloroplast import assays with purified pea (Pisum sativum) chloroplasts and mitochondria Zea mays 9507
-
4.2.1.93 cytosol
-
 Arabidopsis thaliana 5829
-
4.2.1.93 cytosol
-
 Zea mays 5829
-
4.2.1.93 mitochondrion import assays with purified pea (Pisum sativum) chloroplasts and mitochondria Arabidopsis thaliana 5739
-
4.2.1.93 mitochondrion import assays with purified pea (Pisum sativum) chloroplasts and mitochondria Zea mays 5739
-
4.2.1.136 chloroplast
-
 Arabidopsis thaliana 9507
-
4.2.1.136 chloroplast
-
 Zea mays 9507
-
4.2.1.136 cytosol
-
 Arabidopsis thaliana 5829
-
4.2.1.136 cytosol
-
 Zea mays 5829
-
4.2.1.136 mitochondrion
-
 Arabidopsis thaliana 5739
-
4.2.1.136 mitochondrion
-
 Zea mays 5739
-
4.2.1.136 additional information the NAD(P)HX dehydratase co-localizes with an epimerase, that rapidly interconverts (R)- and (S)-NAD(P)HX Arabidopsis thaliana
-
-
4.2.1.136 additional information the NAD(P)HX dehydratase co-localizes with an epimerase, that rapidly interconverts (R)- and (S)-NAD(P)HX Zea mays
-
-
5.1.99.6 chloroplast
-
 Zea mays 9507
-
5.1.99.6 chloroplast
-
 Arabidopsis thaliana 9507
-
5.1.99.6 cytoplasm
-
 Zea mays 5737
-
5.1.99.6 cytoplasm
-
 Arabidopsis thaliana 5737
-
5.1.99.6 mitochondrion
-
 Zea mays 5739
-
5.1.99.6 mitochondrion
-
 Arabidopsis thaliana 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.136 Mg2+ required Arabidopsis thaliana
4.2.1.136 Mg2+ required Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Zea mays stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana Col-0 stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana Columbia-0 stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADPH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Zea mays stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADPH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana Col-0 stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADPH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana Columbia-0 stereospecific for (S)-NAD(P)HX epimer ADP + phosphate + NADPH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana
-
 ADP + phosphate + NADH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Zea mays
-
 ADP + phosphate + NADH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana Col-0
-
 ADP + phosphate + NADH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana Columbia-0
-
 ADP + phosphate + NADH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana
-
 ADP + phosphate + NADPH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Zea mays
-
 ADP + phosphate + NADPH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana Col-0
-
 ADP + phosphate + NADPH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana Columbia-0
-
 ADP + phosphate + NADPH
-
 ?
5.1.99.6 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Zea mays i.e. NADHX (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 r
5.1.99.6 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana i.e. NADHX (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 r
5.1.99.6 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Zea mays i.e. NADPHX (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 r
5.1.99.6 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana i.e. NADPHX (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.93 Arabidopsis thaliana Q94AF2 gene At5g19150
-
4.2.1.93 Arabidopsis thaliana Col-0 Q94AF2 gene At5g19150
-
4.2.1.93 Zea mays A0A3L6F337
-
-
4.2.1.136 Arabidopsis thaliana Q94AF2
-
-
4.2.1.136 Arabidopsis thaliana Columbia-0 Q94AF2
-
-
4.2.1.136 Zea mays A0A3L6F337
-
-
5.1.99.6 Arabidopsis thaliana Q9LTX3
-
-
5.1.99.6 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.136 recombinant N-terminally His-tagged mature dehydratase from Escherichia coli strain BL21 (DE3) RIPL by nickel affinity chromatography Arabidopsis thaliana
5.1.99.6 recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography Zea mays
5.1.99.6 recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.93 seedling
-
 Arabidopsis thaliana
-
4.2.1.136 leaf
-
 Arabidopsis thaliana
-
4.2.1.136 leaf
-
 Zea mays
-
4.2.1.136 seedling
-
 Arabidopsis thaliana
-
5.1.99.6 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana ADP + phosphate + NADH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Zea mays ADP + phosphate + NADH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Col-0 ADP + phosphate + NADH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Columbia-0 ADP + phosphate + NADH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana ADP + phosphate + NADPH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Zea mays ADP + phosphate + NADPH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Col-0 ADP + phosphate + NADPH
-
 ?
4.2.1.93 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate stereospecific for (S)-NAD(P)HX epimer Arabidopsis thaliana Columbia-0 ADP + phosphate + NADPH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 Arabidopsis thaliana ADP + phosphate + NADH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 Zea mays ADP + phosphate + NADH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 Arabidopsis thaliana Col-0 ADP + phosphate + NADH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 Arabidopsis thaliana Columbia-0 ADP + phosphate + NADH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 Arabidopsis thaliana ADP + phosphate + NADPH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 Zea mays ADP + phosphate + NADPH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 Arabidopsis thaliana Col-0 ADP + phosphate + NADPH
-
 ?
4.2.1.136 ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 Arabidopsis thaliana Columbia-0 ADP + phosphate + NADPH
-
 ?
5.1.99.6 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide i.e. NADHX Zea mays (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 r
5.1.99.6 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide i.e. NADHX Arabidopsis thaliana (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 r
5.1.99.6 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate i.e. NADPHX Zea mays (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 r
5.1.99.6 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate i.e. NADPHX Arabidopsis thaliana (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.93 (S)-NAD(P)HX dehydratase
-
 Arabidopsis thaliana
4.2.1.93 (S)-NAD(P)HX dehydratase
-
 Zea mays
4.2.1.136 NAD(P)HX dehydratase
-
 Arabidopsis thaliana
4.2.1.136 NAD(P)HX dehydratase
-
 Zea mays
5.1.99.6 epimerase-PPOX
-
 Zea mays
5.1.99.6 epimerase-PPOX
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.93 22
-
 assay at Arabidopsis thaliana
4.2.1.93 22
-
 assay at Zea mays
4.2.1.136 22
-
 assay at Arabidopsis thaliana
4.2.1.136 22
-
 assay at Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.93 0.26
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana
4.2.1.93 0.3
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
4.2.1.93 0.34
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
4.2.1.93 0.38
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays
4.2.1.136 0.26
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana
4.2.1.136 0.3
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
4.2.1.136 0.34
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
4.2.1.136 0.38
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays
5.1.99.6 0.26
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH and temperature not specified in the publication Arabidopsis thaliana
5.1.99.6 0.38
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH and temperature not specified in the publication Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.93 8
-
 assay at Arabidopsis thaliana
4.2.1.93 8
-
 assay at Zea mays
4.2.1.136 8
-
 assay at Arabidopsis thaliana
4.2.1.136 8
-
 assay at Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.93 ATP dependent on Arabidopsis thaliana
4.2.1.93 ATP dependent on Zea mays
4.2.1.136 ADP dependent on Arabidopsis thaliana
4.2.1.136 ADP dependent on Zea mays
5.1.99.6 additional information the hydratase is dependent on ATP, but the epimerase ist not Zea mays
5.1.99.6 additional information the hydratase is dependent on ATP, but the epimerase ist not Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
4.2.1.93 evolution domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants Arabidopsis thaliana
4.2.1.93 evolution domain architectures of the NAD(P)HX dehydratase enzymes of prokaryotes, yeast, andmammals, and of dehydratase homologue in plants Zea mays
4.2.1.93 physiological function the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites Arabidopsis thaliana
4.2.1.93 physiological function the enzyme is part of a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites Zea mays
4.2.1.136 malfunction ablation of the Arabidopsis dehydratase gene raises seedling levels of all NADHX forms by 20 to 40fold, and levels of one NADPHX form by 10 to 30fold Arabidopsis thaliana
4.2.1.136 metabolism plants have a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites Arabidopsis thaliana
4.2.1.136 metabolism plants have a canonical two-enzyme NAD(P)HX repair system that is directed to three subcellular compartments via the use of alternative translation start sites Zea mays
4.2.1.136 physiological function NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates [NAD(P)HX], which are not electron donors and inhibit various dehydrogenases. The NAD(P)HX dehydratase is able to reconvert (S)-NAD(P)HX to NAD(P)H in an ATP-dependent manner Arabidopsis thaliana
4.2.1.136 physiological function NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates [NAD(P)HX], which are not electron donors and inhibit various dehydrogenases. The NAD(P)HX dehydratase is able to reconvert (S)-NAD(P)HX to NAD(P)H in an ATP-dependent manner Zea mays
5.1.99.6 malfunction a truncated form of the Arabidopsis epimerase lacking the PPOX domain is also fully active in the interconversion Arabidopsis thaliana
5.1.99.6 additional information the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family Zea mays
5.1.99.6 additional information the epimerase homologue is fused to the vitamin B6 salvage enzyme pyridoxine 5'-phosphate oxidase, PPOX. Plant epimerase homologs have an extra C-terminal domain that belongs to the pyridoxine/pyridoxamine phosphate oxidase (PPOX) family Arabidopsis thaliana
5.1.99.6 physiological function NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments Zea mays
5.1.99.6 physiological function NADH and NADPH undergo spontaneous and enzymatic reactions that produce R and S forms of NAD(P)H hydrates, which are not electron donors and inhibit various dehydrogenases. The highly conserved epimerase repairs the hydrates formed from NADH and NADPH in plants together with a highly conserved dehydratase targeted to multiple compartments Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.93 262
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana
4.2.1.93 358
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
4.2.1.93 509
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
4.2.1.93 524
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays
4.2.1.136 262
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Arabidopsis thaliana
4.2.1.136 358
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide pH 8.0, 22°C Zea mays
4.2.1.136 509
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Arabidopsis thaliana
4.2.1.136 524
-
 (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate pH 8.0, 22°C Zea mays