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Literature summary extracted from

  • Hall, D.; Zerbe, P.; Jancsik, S.; Quesada, A.; Dullat, H.; Madilao, L.; Yuen, M.; Bohlmann, J.
    Evolution of conifer diterpene synthases: diterpene resin acid biosynthesis in lodgepole pine and jack pine involves monofunctional and bifunctional diterpene synthases (2013), Plant Physiol., 161, 600-616.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.12 expression of His-tagged enzyme in Escherichia coli Pinus banksiana
5.5.1.12 expression of His-tagged enzyme in Escherichia coli Pinus contorta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.147 (+)-copalyl diphosphate Pinus contorta the enzyme is involved in diterpene biosynthesis pimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.147 (+)-copalyl diphosphate Pinus banksiana
-
 pimara-8(14),15-diene + diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate Pinus banksiana
-
 (+)-copalyl diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate Pinus contorta
-
 (+)-copalyl diphosphate
-
 ?
5.5.1.12 additional information Pinus banksiana gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview ?
-
 ?
5.5.1.12 additional information Pinus contorta gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.B25 Pinus banksiana M4HXW5
-
-
4.2.3.B25 Pinus contorta M4HYP3
-
-
4.2.3.147 Pinus banksiana M4HY08
-
-
4.2.3.147 Pinus contorta M4HYC8
-
-
5.5.1.12 Pinus banksiana M4HXU6 LAS1
-
5.5.1.12 Pinus contorta M4HXW7 class II diTPS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.12 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Pinus banksiana
5.5.1.12 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Pinus contorta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.B25 (+)-copalyl diphosphate monofunctional class I enzyme that lacks functional class II active site Pinus banksiana isopimara-8(14),15-diene + diphosphate products are 88% isopimaradiene and 12% sandaracopimaradiene ?
4.2.3.B25 (+)-copalyl diphosphate monofunctional class I enzyme that lacks functional class II active site Pinus contorta isopimara-8(14),15-diene + diphosphate products are 90% isopimaradiene and up to 10% sandaracopimaradiene ?
4.2.3.B25 additional information no substrate: geranylgeranyl diphosphate Pinus banksiana ?
-
 ?
4.2.3.B25 additional information no substrate: geranylgeranyl diphosphate Pinus contorta ?
-
 ?
4.2.3.147 (+)-copalyl diphosphate the enzyme is involved in diterpene biosynthesis Pinus contorta pimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.147 (+)-copalyl diphosphate
-
 Pinus banksiana pimara-8(14),15-diene + diphosphate
-
 ?
4.2.3.147 (+)-copalyl diphosphate
-
 Pinus contorta pimara-8(14),15-diene + diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate
-
 Pinus banksiana (+)-copalyl diphosphate
-
 ?
5.5.1.12 geranylgeranyl diphosphate
-
 Pinus contorta (+)-copalyl diphosphate
-
 ?
5.5.1.12 additional information gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview Pinus banksiana ?
-
 ?
5.5.1.12 additional information gymnosperm diterpene synthases of diterpene resin acid biosynthesis are bifunctional enzymes that catalyze the initial bicyclization of geranylgeranyl diphosphate followed by rearrangement of a (+)-copalyl diphosphate intermediate at two discrete class II and class I active sites. The bifunctional jack pine and lodgepole pine LAS enzymes demonstrated that the class I active sites of monofunctional proteins can use the (+)-copalyl diphosphate intermediate produced by the class II active site of the bifunctional enzymes, overview Pinus contorta ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.B25 mISO1
-
 Pinus banksiana
4.2.3.B25 mISO1
-
 Pinus contorta
4.2.3.B25 monofunctional isopimaradiene synthase
-
 Pinus contorta
4.2.3.147 monofunctional pimaradiene synthase 1
-
 Pinus contorta
4.2.3.147 PbmPIM1
-
 Pinus banksiana
4.2.3.147 PcmPIM1
-
 Pinus contorta
5.5.1.12 bifunctional LAS-type diTPS
-
 Pinus banksiana
5.5.1.12 bifunctional LAS-type diTPS
-
 Pinus contorta
5.5.1.12 class II diterpene synthase
-
 Pinus banksiana
5.5.1.12 class II diterpene synthase
-
 Pinus contorta
5.5.1.12 class II diTPS
-
 Pinus banksiana
5.5.1.12 class II diTPS
-
 Pinus contorta
5.5.1.12 ISO
-
 Pinus banksiana
5.5.1.12 ISO
-
 Pinus contorta
5.5.1.12 isopimaradiene synthase-type diTPS
-
 Pinus banksiana
5.5.1.12 isopimaradiene synthase-type diTPS
-
 Pinus contorta
5.5.1.12 LAS
-
 Pinus banksiana
5.5.1.12 LAS
-
 Pinus contorta
5.5.1.12 LAS1
-
 Pinus banksiana
5.5.1.12 levopimaradiene/abietadiene synthase-type diTPS
-
 Pinus banksiana
5.5.1.12 levopimaradiene/abietadiene synthase-type diTPS
-
 Pinus contorta

General Information

EC Number General Information Comment Organism
4.2.3.147 metabolism the enzyme is involved in diterpene biosynthesis Pinus contorta
4.2.3.147 metabolism the enzyme is involved in diterpene biosynthesis Pinus banksiana
5.5.1.12 evolution the enzyme belongs to the class II diterpene synthases, phylogenetic tree, diterpene biosynthesis in gymnosperms, overview Pinus banksiana
5.5.1.12 evolution the enzyme belongs to the class II diterpene synthases, phylogenetic tree, diterpene biosynthesis in gymnosperms, overview Pinus contorta
5.5.1.12 metabolism bifunctional class I/II PcLAS1 and PbLAS1 enzymes can supply (+)-copalyl diphosphate for the Monofunctional class I enzymes Pinus banksiana