BRENDA - Enzyme Database

CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism

Wang, Q.; Hillwig, M.L.; Wu, Y.; Peters, R.J.; Plant Physiol. 158, 1418-1425 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.14.70
expressed in Escherichia coli strain C41
Oryza sativa
1.14.14.86
expressed in Escherichia coli BL21(DE3) cells
Oryza sativa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.70
0.002
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
0.0023
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
0.007
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.70
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.70
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.70
ent-sandaracopimaradiene + NADPH + H+ + O2
Oryza sativa
-
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
?
1.14.14.86
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.14.70
Oryza sativa
-
-
-
1.14.14.86
Oryza sativa
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.14.70
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.70
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.70
ent-sandaracopimaradiene + NADPH + H+ + O2
-
728514
Oryza sativa
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.14.86
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.14.70
CYP701A8
-
Oryza sativa
1.14.14.70
KOL4
-
Oryza sativa
1.14.14.86
CYP701A6
-
Oryza sativa
1.14.14.86
KO2
-
Oryza sativa
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.70
0.013
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
0.017
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
0.022
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.70
NADPH
-
Oryza sativa
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.70
expressed in Escherichia coli strain C41
Oryza sativa
1.14.14.86
expressed in Escherichia coli BL21(DE3) cells
Oryza sativa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.70
NADPH
-
Oryza sativa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.70
0.002
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
0.0023
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
0.007
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.14.70
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.70
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.70
ent-sandaracopimaradiene + NADPH + H+ + O2
Oryza sativa
-
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
?
1.14.14.86
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa
-
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.14.70
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
3alpha-hydroxy-ent-cassadiene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.70
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
3alpha-hydroxy-ent-kaurene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.70
ent-sandaracopimaradiene + NADPH + H+ + O2
-
728514
Oryza sativa
ent-sandaracopimaradien-3beta-ol + NADP+ + H2O
-
-
-
?
1.14.14.86
ent-kaurene + [reduced NADPH-hemoprotein reductase] + O2
-
728514
Oryza sativa
(-)-kaurenoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.14.70
0.013
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
0.017
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
0.022
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.14.14.70
3
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
6
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
9
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.14.14.70
3
-
ent-kaurene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
6
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication
Oryza sativa
1.14.14.70
9
-
ent-cassadiene
pH and temperature not specified in the publication
Oryza sativa