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Literature summary extracted from

  • Foster, J.; Kim, H.U.; Nakata, P.A.; Browse, J.
    A previously unknown oxalyl-CoA synthetase is important for oxalate catabolism in Arabidopsis (2012), Plant Cell, 24, 1217-1229.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.8 expression of GFP-tagged enzyme in onion epidermal cell cytoplasm using the constitutive 35S promoter, and from binary construct, transfected into Agrobacterium tumefaciens, GFP-tagged enzyme is transiently expressed in Nicotiana benthamiana leaf cytoplasm Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.8 additional information aae3-1 and aae3-2 are null mutants are deficient in oxalyl-CoA synthetase Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.8 0.149
-
 oxalate pH 8.0, temperature not specified in the publication Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.8 cytoplasm
-
 Arabidopsis thaliana 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.8 ATP + oxalate + CoA Arabidopsis thaliana
-
 AMP + diphosphate + oxalyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.8 Arabidopsis thaliana Q9SMT7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.8 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.8 ATP + oxalate + CoA
-
 Arabidopsis thaliana AMP + diphosphate + oxalyl-CoA
-
 ?
6.2.1.8 ATP + oxalate + CoA specific for oxalate Arabidopsis thaliana AMP + diphosphate + oxalyl-CoA
-
 ?
6.2.1.8 additional information no activity with malonate, succinate, malate, acetate, formate, lactate, glycolate, glyoxylate, and glutarate Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.8 AAE3
-
 Arabidopsis thaliana
6.2.1.8 acyl-activating enzyme3
-
 Arabidopsis thaliana
6.2.1.8 Oxalyl-CoA synthetase
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.8 8
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.8 5 10 activity range, profile overview Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.8 ATP
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
6.2.1.8 malfunction aae3 null mutants show increased soluble and insoluble oxalate contents compared to the wild-type enzyme, loss of AAE3 interferes with seed development affecting the integrity and function of the seed coat, phenotype, overview Arabidopsis thaliana
6.2.1.8 metabolism the enzyme catalyzes the first step in oxalate degradation Arabidopsis thaliana
6.2.1.8 physiological function oxalyl-CoA synthetase is required for oxalate degradation, for normal seed development, and for defense against an oxalate-producing fungal pathogen, e.g. the fungus Sclerotinia sclerotiorum Arabidopsis thaliana