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Literature summary extracted from

  • Singh, K.K.; Ghosh, S.
    Regulation of glutamine synthetase isoforms in two differentially drought-tolerant rice (Oryza sativa L.) cultivars under water deficit conditions (2013), Plant Cell Rep., 32, 183-193.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 chloroplast isozyme GS2 Oryza sativa 9507
-
6.3.1.2 cytosol isozyme OsGS1;1 Oryza sativa Japonica Group 5829
-
6.3.1.2 cytosol isozyme OsGS1;2 Oryza sativa 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ required Oryza sativa
6.3.1.2 Mg2+ required Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3 Oryza sativa
-
 ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + L-glutamate + NH3 Oryza sativa Japonica Group
-
 ADP + phosphate + L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Oryza sativa
-
 var. indica, drought-sensitive IR-64 cultivar and drought-tolerant Khitish cultivar, gene OsGS1;2
-
6.3.1.2 Oryza sativa P14655 OsGS2; var. indica, drought-sensitive IR-64 cultivar and drought-tolerant Khitish cultivar, gene OsGS2
-
6.3.1.2 Oryza sativa Japonica Group P14656 OsGS1;1; var. indica, drought-sensitive IR-64 cultivar and drought-tolerant Khitish cultivar, gene OsGS1;1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.2 leaf
-
 Oryza sativa
-
6.3.1.2 leaf
-
 Oryza sativa Japonica Group
-
6.3.1.2 additional information mRNA and protein contents of GS isoforms, overview Oryza sativa
-
6.3.1.2 additional information mRNA and protein contents of GS isoforms, overview Oryza sativa Japonica Group
-
6.3.1.2 root
-
 Oryza sativa
-
6.3.1.2 root
-
 Oryza sativa Japonica Group
-
6.3.1.2 seedling
-
 Oryza sativa
-
6.3.1.2 seedling
-
 Oryza sativa Japonica Group
-
6.3.1.2 stem
-
 Oryza sativa
-
6.3.1.2 stem
-
 Oryza sativa Japonica Group
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 additional information
-
 total GS, GS1 and GS2 activities in leaf, stem and root of rice in cultivars IR-64 and Khitish seedlings at various stages of water deprivation, overview Oryza sativa
6.3.1.2 additional information
-
 total GS, GS1 and GS2 activities in leaf, stem and root of rice in cultivars IR-64 and Khitish seedlings at various stages of water deprivation, overview Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3
-
 Oryza sativa ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 ATP + L-glutamate + NH3
-
 Oryza sativa Japonica Group ADP + phosphate + L-glutamine
-
 ?
6.3.1.2 hydroxylamine + L-glutamine + ATP gamma-glutamylhydroxamate synthetase activity Oryza sativa L-gamma-glutamyl-hydroxamate + ammonia + ADP
-
 ?
6.3.1.2 hydroxylamine + L-glutamine + ATP gamma-glutamylhydroxamate synthetase activity Oryza sativa Japonica Group L-gamma-glutamyl-hydroxamate + ammonia + ADP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 37
-
 assay at Oryza sativa
6.3.1.2 37
-
 assay at Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.5
-
 assay at Oryza sativa
6.3.1.2 7.5
-
 assay at Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.2 ATP
-
 Oryza sativa
6.3.1.2 ATP
-
 Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
6.3.1.2 Oryza sativa Japonica Group under drought stress, total GS activity in root and leaf decreases significantly in IR-64 seedlings in comparison to Khitish seedlings. The reduced GS activity in IR-64 leaf is mainly due to decrease in isozyme GS2 activity down
6.3.1.2 Oryza sativa under drought stress, total GS activity in root and leaf decreases significantly in IR-64 seedlings in comparison to Khitish seedlings. The reduced GS activity in IR-64 leaf is mainly due to decrease in isozyme GS2 activity, which correlates with decrease in corresponding transcript and polypeptide contents down
6.3.1.2 Oryza sativa total enzyme activity in stem of both the varieties is insensitive to drought additional information
6.3.1.2 Oryza sativa Japonica Group total enzyme activity in stem of both the varieties is insensitive to drought additional information
6.3.1.2 Oryza sativa gene GS1 transcript and polypeptide accumulate in leaf during drought, but GS1 activity is maintained at a constant level up
6.3.1.2 Oryza sativa Japonica Group gene GS1 transcript and polypeptide accumulate in leaf during drought, but GS1 activity is maintained at a constant level up

General Information

EC Number General Information Comment Organism
6.3.1.2 physiological function the enzyme catalyzes the ATP-dependent assimilation of ammonium into glutamate to yield glutamine, which is then used for the biosynthesis of essentially all nitrogenous compounds. Effect of water deprivation varies with variety, degree and duration of stress Oryza sativa
6.3.1.2 physiological function the enzyme catalyzes the ATP-dependent assimilation of ammonium into glutamate to yield glutamine, which is then used for the biosynthesis of essentially all nitrogenous compounds. Gene OsGS1;1 expression is differently regulated by drought stresss in the two rice Oryza sativa varieties, effect of water deprivation varies with variety, degree and duration of stress Oryza sativa Japonica Group