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Literature summary extracted from

  • Chang, C.H.; Wen, H.Y.; Shie, W.S.; Lu, C.T.; Li, M.E.; Liu, Y.T.; Li, W.H.; Wu, T.K.
    Protein engineering of oxidosqualene-lanosterol cyclase into triterpene monocyclase (2013), Org. Biomol. Chem., 11, 4214-4219.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.7 C457G site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 C457G/T509G site-directed mutagenesis, the mutations disrupt the pre-existing H-bond to the protonating Asp456 and the intrinsic His234-Tyr510 H-bond network, respectively, and generates achilleol A as the major product Saccharomyces cerevisiae
5.4.99.7 H234W/Y510W site-directed mutagenesis, the double mutation alters the ERG7 function to achilleol A synthase activity and generates achilleol A as the sole product Saccharomyces cerevisiae
5.4.99.7 T509G site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 W587F site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 W587Y site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 Y510A site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 Y510C site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 Y510H site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 Y510K site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 Y510L site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 Y510S site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae
5.4.99.7 Y510W site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene Saccharomyces cerevisiae
-
lanosterol
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.7 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene
-
Saccharomyces cerevisiae lanosterol
-
?
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene reaction of wild-type enzyme Saccharomyces cerevisiae lanosterol
-
?
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene reaction of mutant H234W/Y510W Saccharomyces cerevisiae achilleol A
-
?
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene reaction of C457G/T509G Saccharomyces cerevisiae lanosterol + achilleol A achilleol A is the main product ?
5.4.99.7 (3S)-2,3-epoxy-2,3-dihydrosqualene reaction of mutant H234W/Y510V Saccharomyces cerevisiae lanosterol + achilleol A + 13alphaH-isomalabarica-14(26),17E,21-trien-3beta-ol lanosterol is the main product ?
5.4.99.7 additional information mutant enzyme display altered product profiles compared to the wild-type enzyme producing also e.g. achilleol A, camelliol C, 13alphaH-isomalabarica-14(26),17E,21-trien-3beta-ol, protosta-12(13),24-dien-3beta-ol, protosta-13(17),24-dien-3beta-ol, or parkeol, overview Saccharomyces cerevisiae ?
-
?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.7 ERG7
-
Saccharomyces cerevisiae
5.4.99.7 Oxidosqualene-lanosterol cyclase
-
Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
5.4.99.7 additional information structure-function-mechanism relationships, overview Saccharomyces cerevisiae