EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.7 | C457G | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | C457G/T509G | site-directed mutagenesis, the mutations disrupt the pre-existing H-bond to the protonating Asp456 and the intrinsic His234-Tyr510 H-bond network, respectively, and generates achilleol A as the major product | Saccharomyces cerevisiae |
5.4.99.7 | H234W/Y510W | site-directed mutagenesis, the double mutation alters the ERG7 function to achilleol A synthase activity and generates achilleol A as the sole product | Saccharomyces cerevisiae |
5.4.99.7 | T509G | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | W587F | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | W587Y | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | Y510A | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | Y510C | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | Y510H | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | Y510K | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | Y510L | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | Y510S | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
5.4.99.7 | Y510W | site-directed mutagenesis, the mutant shows an altered product profile compared to the wild-type enzyme, overview | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Saccharomyces cerevisiae | - |
lanosterol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.7 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Saccharomyces cerevisiae | lanosterol | - |
? | |
5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of wild-type enzyme | Saccharomyces cerevisiae | lanosterol | - |
? | |
5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant H234W/Y510W | Saccharomyces cerevisiae | achilleol A | - |
? | |
5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of C457G/T509G | Saccharomyces cerevisiae | lanosterol + achilleol A | achilleol A is the main product | ? | |
5.4.99.7 | (3S)-2,3-epoxy-2,3-dihydrosqualene | reaction of mutant H234W/Y510V | Saccharomyces cerevisiae | lanosterol + achilleol A + 13alphaH-isomalabarica-14(26),17E,21-trien-3beta-ol | lanosterol is the main product | ? | |
5.4.99.7 | additional information | mutant enzyme display altered product profiles compared to the wild-type enzyme producing also e.g. achilleol A, camelliol C, 13alphaH-isomalabarica-14(26),17E,21-trien-3beta-ol, protosta-12(13),24-dien-3beta-ol, protosta-13(17),24-dien-3beta-ol, or parkeol, overview | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.7 | ERG7 | - |
Saccharomyces cerevisiae |
5.4.99.7 | Oxidosqualene-lanosterol cyclase | - |
Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.4.99.7 | additional information | structure-function-mechanism relationships, overview | Saccharomyces cerevisiae |