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Literature summary extracted from

  • Kobayashi, T.; Nureki, O.; Ishitani, R.; Yaremchuk, A.; Tukalo, M.; Cusack, S.;, Sakamoto K, Yokoyama S.
    Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion (2003), Nat. Struct. Biol., 10, 425-432.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.1 structure of the TyrRS-tRNA(Tyr)-L-tyrosine complex, solved at a resolution of 1.95 A Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.1 D286R the mutant enzyme aminoacylates the amber suppressor tRNA, as well as the wild-type tRNATyr, whereas the wild-type enzyme aminoacylates the amber suppresssor tRNA about 300fold less efficientyl than the wild-type tRNATyr. The mutant recognizes the amber suppressor tRNA 65fold better than the wild-type enzyme. The activity if the mutant and amber suppressor tRNA pair is as high as 22% that of the wild-type pair. The mutation mainly decreases the KM for tRNA. The kcat is not affected as much Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.1 0.00035
-
 tRNATyr(wild-type) 37°C, pH 7.5, wild-type enzyme Methanocaldococcus jannaschii
6.1.1.1 0.00068
-
 tRNATyr(G34C) 37°C, pH 7.5, mutant enzyme D268R Methanocaldococcus jannaschii
6.1.1.1 0.0014
-
 tRNATyr(wild-type) 37°C, pH 7.5, mutant enzyme D268R Methanocaldococcus jannaschii
6.1.1.1 0.039
-
 tRNATyr(G34C) 37°C, pH 7.5, wild-type enzyme Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii DSM 2661
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.1 Methanocaldococcus jannaschii Q57834
-
-
6.1.1.1 Methanocaldococcus jannaschii DSM 2661 Q57834
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr(G34C) the enzyme strictly recognizes the C1-G72 base pair, whereas the enzyme from Thermus thermophilus recognizes the G1-C72 in a different manner using different residues Methanocaldococcus jannaschii AMP + diphosphate + L-tyrosyl-tRNATyr(G34C)
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr(G34C) the enzyme strictly recognizes the C1-G72 base pair, whereas the enzyme from Thermus thermophilus recognizes the G1-C72 in a different manner using different residues Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + L-tyrosyl-tRNATyr(G34C)
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr(wild-type) the enzyme strictly recognizes the C1-G72 base pair, whereas the enzyme from Thermus thermophilus recognizes the G1-C72 in a different manner using different residues Methanocaldococcus jannaschii AMP + diphosphate + L-tyrosyl-tRNATyr(wild-type)
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr(wild-type) the enzyme strictly recognizes the C1-G72 base pair, whereas the enzyme from Thermus thermophilus recognizes the G1-C72 in a different manner using different residues Methanocaldococcus jannaschii DSM 2661 AMP + diphosphate + L-tyrosyl-tRNATyr(wild-type)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.1 TyrRS
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.1 37
-
 assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.1 0.07
-
 tRNATyr(G34C) 37°C, pH 7.5, wild-type enzyme Methanocaldococcus jannaschii
6.1.1.1 0.079
-
 tRNATyr(G34C) 37°C, pH 7.5, mutant enzyme D268R Methanocaldococcus jannaschii
6.1.1.1 0.12
-
 tRNATyr(wild-type) 37°C, pH 7.5, mutant enzyme D268R Methanocaldococcus jannaschii
6.1.1.1 0.19
-
 tRNATyr(wild-type) 37°C, pH 7.5, wild-type enzyme Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.1 7.5
-
 assay at Methanocaldococcus jannaschii