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Literature summary extracted from

  • Zaffagnini, M.; Michelet, L.; Sciabolini, C.; Di Giacinto, N.; Morisse, S.; Marchand, C.H.; Trost, P.; Fermani, S.; Lemaire, S.D.
    High-resolution crystal structure and redox properties of chloroplastic triosephosphate isomerase from Chlamydomonas reinhardtii (2014), Mol. Plant, 7, 101-120.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.1 expressed in Escherichia coli BL21 cells Chlamydomonas reinhardtii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.1 sitting drop vapor diffusion method, using either 65% (v/v) 2-methyl-2,4-pentanediol 0.1 M HEPES, pH 7.5, or 6% (w/v) PEG 6000 0.1 M HEPES, pH 7.0, or 17.5% (w/v) PEG 3350, 0.1 M Bis-Tris, pH 5.5, or 15% (w/v) PEG 3350 0.1 M Bis-Tris, pH 5.0 Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.1 H2O2 the inhibition of the enzyme is negligible when incubated with 0.1 mM H2O2, whereas a 10% inhibition is observed with 1 mM H2O2 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.1 3.04
-
D-glyceraldehyde 3-phosphate in 100 mM triethanolamine (pH 7.4), at 25°C Chlamydomonas reinhardtii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.1.1 chloroplast
-
Chlamydomonas reinhardtii 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.1 28249
-
2 * 28249, calculated from amino acid sequence Chlamydomonas reinhardtii
5.3.1.1 28266
-
2 * 28266, MALDI-TOF mass spectrometry Chlamydomonas reinhardtii
5.3.1.1 58800
-
gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.1 D-Glyceraldehyde 3-phosphate Chlamydomonas reinhardtii
-
Glycerone phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.1 Chlamydomonas reinhardtii Q5S7Y5
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
5.3.1.1 the inhibition of the enzyme is negligible when incubated with 0.1 mM H2O2, whereas a 10% inhibition is observed with 1 mM H2O2 Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.1 Ni2+ HiTrap chelating resin column chromatography Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.1 7193
-
in 100 mM triethanolamine (pH 7.4), at 25°C Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.1 D-Glyceraldehyde 3-phosphate
-
Chlamydomonas reinhardtii Glycerone phosphate
-
r

Subunits

EC Number Subunits Comment Organism
5.3.1.1 homodimer 2 * 28249, calculated from amino acid sequence Chlamydomonas reinhardtii
5.3.1.1 homodimer 2 * 28266, MALDI-TOF mass spectrometry Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
5.3.1.1 TpI
-
Chlamydomonas reinhardtii
5.3.1.1 Triosephosphate isomerase
-
Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.1 3372
-
D-glyceraldehyde 3-phosphate in 100 mM triethanolamine (pH 7.4), at 25°C Chlamydomonas reinhardtii

General Information

EC Number General Information Comment Organism
5.3.1.1 metabolism the chloroplastic isoform participates in the Calvin-Benson cycle Chlamydomonas reinhardtii