EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.71 | a hexahistidine-tagged recombinant version of the enzyme is heterologously overexpressed in Haloferax volcanii | Halobacterium salinarum |
1.1.1.71 | expresssion in Haloferax volcanii | Halobacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.71 | 0.05 | - |
NADPH | pH 6.0, 50°C | Halobacterium salinarum | |
1.1.1.71 | 0.05 | - |
NADPH | pH 6.0, 50°C | Halobacterium sp. | |
1.1.1.71 | 0.2 | - |
NADP+ | pH 6.0, 50°C | Halobacterium sp. | |
1.1.1.71 | 0.2 | - |
NADP+ | pH 10.0, 50°C | Halobacterium salinarum | |
1.1.1.71 | 3.4 | - |
acetaldehyde | pH 6.0, 50°C | Halobacterium salinarum | |
1.1.1.71 | 3.4 | - |
acetaldehyde | pH 6.0, 50°C | Halobacterium sp. | |
1.1.1.71 | 21.4 | - |
1-propanol | pH 6.0, 50°C | Halobacterium sp. | |
1.1.1.71 | 21.4 | - |
1-propanol | pH 10.0, 50°C | Halobacterium salinarum | |
1.1.1.71 | 107.9 | - |
ethanol | pH 6.0, 50°C | Halobacterium sp. | |
1.1.1.71 | 107.9 | - |
ethanol | pH 10.0, 50°C | Halobacterium salinarum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.71 | KCl | a preference for KCl over NaCl is observed, with best activity at 4 M KCl | Halobacterium salinarum | |
1.1.1.71 | KCl | maximum activity in presence of 4 M KCl | Halobacterium sp. | |
1.1.1.71 | NaCl | the enzyme requires high salt concentrations for its activity, a preference for KCl over NaCl is observed | Halobacterium salinarum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.71 | 37300 | - |
x * 37300, calculated and SDS-PAGE | Halobacterium sp. |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.1.1.71 | acetonitrile | 24 h at 4°C, 13% loss of activity at 5% (v/v), 10% loss of activity at 10% (v/v), complete loss of activity at 30% (v/v) | Halobacterium salinarum |
1.1.1.71 | acetonitrile | 10%, 90% residual activity | Halobacterium sp. |
1.1.1.71 | dimethyl sulfoxide | 24 h at 4°C, 11% loss of activity at 5% (v/v), 9% loss of activity at 10% (v/v), 59% loss of activity at 30% (v/v) | Halobacterium salinarum |
1.1.1.71 | DMSO | 10%, 91% residual activity | Halobacterium sp. |
1.1.1.71 | Methanol | 24 h at 4°C, 8% loss of activity at 5% (v/v), 15% loss of activity at 10% (v/v), 94% loss of activity at 30% (v/v) | Halobacterium salinarum |
1.1.1.71 | Methanol | 10%, 85% residual activity | Halobacterium sp. |
1.1.1.71 | tetrahydrofuran | 24 h at 4°C, 37% loss of activity at 5% (v/v), 57% loss of activity at 10% (v/v), complete loss of activity at 30% (v/v) | Halobacterium salinarum |
1.1.1.71 | tetrahydrofuran | 10%, 43% residual activity | Halobacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.71 | Halobacterium salinarum | Q9HMB6 | - |
- |
1.1.1.71 | Halobacterium salinarum NRC 1 | Q9HMB6 | - |
- |
1.1.1.71 | Halobacterium sp. | Q9HMB6 | - |
- |
1.1.1.71 | Halobacterium sp. NRC-1 | Q9HMB6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.71 | purified in one step by immobilised Ni-affinity chromatography | Halobacterium salinarum |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.71 | -20°C, 30 days, 80% residual activity | Halobacterium sp. |
1.1.1.71 | -20°C, 30 days, enzyme retains 80% of its original activity | Halobacterium salinarum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.71 | 1-butanol + NADP+ | 80% compared to activity with 1-propanol | Halobacterium salinarum | butanal + NADPH + H+ | - |
? | |
1.1.1.71 | 1-butanol + NADP+ | 80% of the activtiy with 1-propanol | Halobacterium sp. | butanal + NADPH + H+ | - |
? | |
1.1.1.71 | 1-butanol + NADP+ | 80% compared to activity with 1-propanol | Halobacterium salinarum NRC 1 | butanal + NADPH + H+ | - |
? | |
1.1.1.71 | 1-butanol + NADP+ | 80% of the activtiy with 1-propanol | Halobacterium sp. NRC-1 | butanal + NADPH + H+ | - |
? | |
1.1.1.71 | 1-phenylethanol + NADP+ | 35% compared to activity with 1-propanol | Halobacterium salinarum | acetophenone + NADPH + H+ | - |
? | |
1.1.1.71 | 1-phenylethanol + NADP+ | 35% of the activtiy with 1-propanol | Halobacterium sp. | 1-phenylethanone + NADPH + H+ | - |
? | |
1.1.1.71 | 1-propanol + NAD+ | dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ | Halobacterium salinarum | propanal + NADH + H+ | - |
? | |
1.1.1.71 | 1-propanol + NAD+ | dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ | Halobacterium salinarum NRC 1 | propanal + NADH + H+ | - |
? | |
1.1.1.71 | 1-propanol + NADP+ | - |
Halobacterium sp. | propanal + NADPH + H+ | - |
? | |
1.1.1.71 | 1-propanol + NADP+ | dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ | Halobacterium salinarum | propanal + NADPH + H+ | - |
? | |
1.1.1.71 | 1-propanol + NADP+ | - |
Halobacterium sp. NRC-1 | propanal + NADPH + H+ | - |
? | |
1.1.1.71 | 2-butanol + NADP+ | 30% compared to activity with 1-propanol | Halobacterium salinarum | butanone + NADPH + H+ | - |
? | |
1.1.1.71 | 2-butanol + NADP+ | 30% of the activtiy with 1-propanol | Halobacterium sp. | butanone + NADPH + H+ | - |
? | |
1.1.1.71 | 2-propanol + NADP+ | 25% compared to activity with 1-propanol | Halobacterium salinarum | acetone + NADPH + H+ | - |
? | |
1.1.1.71 | 2-propanol + NADP+ | 25% compared to activity with 1-propanol | Halobacterium salinarum NRC 1 | acetone + NADPH + H+ | - |
? | |
1.1.1.71 | acetaldehyde + NADPH + H+ | - |
Halobacterium sp. | ethanol + NADP+ | - |
? | |
1.1.1.71 | acetaldehyde + NADPH + H+ | - |
Halobacterium sp. NRC-1 | ethanol + NADP+ | - |
? | |
1.1.1.71 | benzyl alcohol + NADP+ | 20% compared to activity with 1-propanol | Halobacterium salinarum | benzaldehyde + NADPH + H+ | - |
? | |
1.1.1.71 | ethanol + NADP+ | 85% compared to activity with 1-propanol | Halobacterium salinarum | acetaldehyde + NADPH + H+ | - |
? | |
1.1.1.71 | ethanol + NADP+ | 84% of the activtiy with 1-propanol | Halobacterium sp. | acetaldehyde + NADPH + H+ | - |
? | |
1.1.1.71 | ethanol + NADP+ | 85% compared to activity with 1-propanol | Halobacterium salinarum NRC 1 | acetaldehyde + NADPH + H+ | - |
? | |
1.1.1.71 | ethanol + NADP+ | 84% of the activtiy with 1-propanol | Halobacterium sp. NRC-1 | acetaldehyde + NADPH + H+ | - |
? | |
1.1.1.71 | additional information | preference for short-chain alcohol substrates, particularly for 1-propanol (100%) and ethanol (84%), no activity with glycerol or methanol | Halobacterium salinarum | ? | - |
? | |
1.1.1.71 | additional information | no substrates: methanol, glycerol | Halobacterium sp. | ? | - |
? | |
1.1.1.71 | additional information | preference for short-chain alcohol substrates, particularly for 1-propanol (100%) and ethanol (84%), no activity with glycerol or methanol | Halobacterium salinarum NRC 1 | ? | - |
? | |
1.1.1.71 | additional information | no substrates: methanol, glycerol | Halobacterium sp. NRC-1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.71 | ? | x * 37300, calculated and SDS-PAGE | Halobacterium sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.71 | ADH2 | - |
Halobacterium salinarum |
1.1.1.71 | ADH2 | - |
Halobacterium sp. |
1.1.1.71 | VNG_2617G | locus name | Halobacterium salinarum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.71 | 55 | 60 | oxidation of alcohol | Halobacterium salinarum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.71 | 6 | - |
reduction of aldehyde | Halobacterium salinarum |
1.1.1.71 | 6 | - |
reduction of aldehydes | Halobacterium sp. |
1.1.1.71 | 10 | - |
oxidation of alcohol | Halobacterium salinarum |
1.1.1.71 | 10 | - |
oxidation of alcohols | Halobacterium sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.71 | NAD+ | dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ | Halobacterium salinarum | |
1.1.1.71 | NADH | about 60% of the activity with NADPH | Halobacterium sp. | |
1.1.1.71 | NADP+ | dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ | Halobacterium salinarum | |
1.1.1.71 | NADPH | preferred cofactor | Halobacterium sp. |