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Literature summary extracted from

  • Liliensiek, A.K.; Cassidy, J.; Gucciardo, G.; Whitely, C.; Paradisi, F.
    Heterologous overexpression, purification and characterisation of an alcohol dehydrogenase (ADH2) from Halobacterium sp. NRC-1 (2013), Mol. Biotechnol., 55, 143-149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.71 a hexahistidine-tagged recombinant version of the enzyme is heterologously overexpressed in Haloferax volcanii Halobacterium salinarum
1.1.1.71 expresssion in Haloferax volcanii Halobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.71 0.05
-
NADPH pH 6.0, 50°C Halobacterium salinarum
1.1.1.71 0.05
-
NADPH pH 6.0, 50°C Halobacterium sp.
1.1.1.71 0.2
-
NADP+ pH 6.0, 50°C Halobacterium sp.
1.1.1.71 0.2
-
NADP+ pH 10.0, 50°C Halobacterium salinarum
1.1.1.71 3.4
-
acetaldehyde pH 6.0, 50°C Halobacterium salinarum
1.1.1.71 3.4
-
acetaldehyde pH 6.0, 50°C Halobacterium sp.
1.1.1.71 21.4
-
1-propanol pH 6.0, 50°C Halobacterium sp.
1.1.1.71 21.4
-
1-propanol pH 10.0, 50°C Halobacterium salinarum
1.1.1.71 107.9
-
ethanol pH 6.0, 50°C Halobacterium sp.
1.1.1.71 107.9
-
ethanol pH 10.0, 50°C Halobacterium salinarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.71 KCl a preference for KCl over NaCl is observed, with best activity at 4 M KCl Halobacterium salinarum
1.1.1.71 KCl maximum activity in presence of 4 M KCl Halobacterium sp.
1.1.1.71 NaCl the enzyme requires high salt concentrations for its activity, a preference for KCl over NaCl is observed Halobacterium salinarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.71 37300
-
x * 37300, calculated and SDS-PAGE Halobacterium sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.71 acetonitrile 24 h at 4°C, 13% loss of activity at 5% (v/v), 10% loss of activity at 10% (v/v), complete loss of activity at 30% (v/v) Halobacterium salinarum
1.1.1.71 acetonitrile 10%, 90% residual activity Halobacterium sp.
1.1.1.71 dimethyl sulfoxide 24 h at 4°C, 11% loss of activity at 5% (v/v), 9% loss of activity at 10% (v/v), 59% loss of activity at 30% (v/v) Halobacterium salinarum
1.1.1.71 DMSO 10%, 91% residual activity Halobacterium sp.
1.1.1.71 Methanol 24 h at 4°C, 8% loss of activity at 5% (v/v), 15% loss of activity at 10% (v/v), 94% loss of activity at 30% (v/v) Halobacterium salinarum
1.1.1.71 Methanol 10%, 85% residual activity Halobacterium sp.
1.1.1.71 tetrahydrofuran 24 h at 4°C, 37% loss of activity at 5% (v/v), 57% loss of activity at 10% (v/v), complete loss of activity at 30% (v/v) Halobacterium salinarum
1.1.1.71 tetrahydrofuran 10%, 43% residual activity Halobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.71 Halobacterium salinarum Q9HMB6
-
-
1.1.1.71 Halobacterium salinarum NRC 1 Q9HMB6
-
-
1.1.1.71 Halobacterium sp. Q9HMB6
-
-
1.1.1.71 Halobacterium sp. NRC-1 Q9HMB6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.71 purified in one step by immobilised Ni-affinity chromatography Halobacterium salinarum

Storage Stability

EC Number Storage Stability Organism
1.1.1.71 -20°C, 30 days, 80% residual activity Halobacterium sp.
1.1.1.71 -20°C, 30 days, enzyme retains 80% of its original activity Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.71 1-butanol + NADP+ 80% compared to activity with 1-propanol Halobacterium salinarum butanal + NADPH + H+
-
?
1.1.1.71 1-butanol + NADP+ 80% of the activtiy with 1-propanol Halobacterium sp. butanal + NADPH + H+
-
?
1.1.1.71 1-butanol + NADP+ 80% compared to activity with 1-propanol Halobacterium salinarum NRC 1 butanal + NADPH + H+
-
?
1.1.1.71 1-butanol + NADP+ 80% of the activtiy with 1-propanol Halobacterium sp. NRC-1 butanal + NADPH + H+
-
?
1.1.1.71 1-phenylethanol + NADP+ 35% compared to activity with 1-propanol Halobacterium salinarum acetophenone + NADPH + H+
-
?
1.1.1.71 1-phenylethanol + NADP+ 35% of the activtiy with 1-propanol Halobacterium sp. 1-phenylethanone + NADPH + H+
-
?
1.1.1.71 1-propanol + NAD+ dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ Halobacterium salinarum propanal + NADH + H+
-
?
1.1.1.71 1-propanol + NAD+ dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ Halobacterium salinarum NRC 1 propanal + NADH + H+
-
?
1.1.1.71 1-propanol + NADP+
-
Halobacterium sp. propanal + NADPH + H+
-
?
1.1.1.71 1-propanol + NADP+ dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ Halobacterium salinarum propanal + NADPH + H+
-
?
1.1.1.71 1-propanol + NADP+
-
Halobacterium sp. NRC-1 propanal + NADPH + H+
-
?
1.1.1.71 2-butanol + NADP+ 30% compared to activity with 1-propanol Halobacterium salinarum butanone + NADPH + H+
-
?
1.1.1.71 2-butanol + NADP+ 30% of the activtiy with 1-propanol Halobacterium sp. butanone + NADPH + H+
-
?
1.1.1.71 2-propanol + NADP+ 25% compared to activity with 1-propanol Halobacterium salinarum acetone + NADPH + H+
-
?
1.1.1.71 2-propanol + NADP+ 25% compared to activity with 1-propanol Halobacterium salinarum NRC 1 acetone + NADPH + H+
-
?
1.1.1.71 acetaldehyde + NADPH + H+
-
Halobacterium sp. ethanol + NADP+
-
?
1.1.1.71 acetaldehyde + NADPH + H+
-
Halobacterium sp. NRC-1 ethanol + NADP+
-
?
1.1.1.71 benzyl alcohol + NADP+ 20% compared to activity with 1-propanol Halobacterium salinarum benzaldehyde + NADPH + H+
-
?
1.1.1.71 ethanol + NADP+ 85% compared to activity with 1-propanol Halobacterium salinarum acetaldehyde + NADPH + H+
-
?
1.1.1.71 ethanol + NADP+ 84% of the activtiy with 1-propanol Halobacterium sp. acetaldehyde + NADPH + H+
-
?
1.1.1.71 ethanol + NADP+ 85% compared to activity with 1-propanol Halobacterium salinarum NRC 1 acetaldehyde + NADPH + H+
-
?
1.1.1.71 ethanol + NADP+ 84% of the activtiy with 1-propanol Halobacterium sp. NRC-1 acetaldehyde + NADPH + H+
-
?
1.1.1.71 additional information preference for short-chain alcohol substrates, particularly for 1-propanol (100%) and ethanol (84%), no activity with glycerol or methanol Halobacterium salinarum ?
-
?
1.1.1.71 additional information no substrates: methanol, glycerol Halobacterium sp. ?
-
?
1.1.1.71 additional information preference for short-chain alcohol substrates, particularly for 1-propanol (100%) and ethanol (84%), no activity with glycerol or methanol Halobacterium salinarum NRC 1 ?
-
?
1.1.1.71 additional information no substrates: methanol, glycerol Halobacterium sp. NRC-1 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.71 ? x * 37300, calculated and SDS-PAGE Halobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.71 ADH2
-
Halobacterium salinarum
1.1.1.71 ADH2
-
Halobacterium sp.
1.1.1.71 VNG_2617G locus name Halobacterium salinarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.71 55 60 oxidation of alcohol Halobacterium salinarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.71 6
-
reduction of aldehyde Halobacterium salinarum
1.1.1.71 6
-
reduction of aldehydes Halobacterium sp.
1.1.1.71 10
-
oxidation of alcohol Halobacterium salinarum
1.1.1.71 10
-
oxidation of alcohols Halobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.71 NAD+ dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ Halobacterium salinarum
1.1.1.71 NADH about 60% of the activity with NADPH Halobacterium sp.
1.1.1.71 NADP+ dual cofactor dependency, NAD+ shows 60% of the activity with NADP+ Halobacterium salinarum
1.1.1.71 NADPH preferred cofactor Halobacterium sp.