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Literature summary extracted from

  • Cho, Y.; Kim, J.; Kim, D.
    Cobalt-requiring 5'-deoxy-5'-methylthioadenosine nucleosidase from soybean (Glycine max) cotyledon (2012), J. Plant Biochem. Biotechnol., 21, 113-116.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.16 5,5'-dithio-bis(2-nitrobenzoic acid)
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.16 additional information
-
 additional information Michaelis-Menten kinetics Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.16 Co2+ requires Co2+ for activity. Cobalt is bound to the nucleosidase with a stoichiometry of 1 equivalent of cobalt/subunit Glycine max
3.2.2.16 Co2+ required for catalytic function Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.16 59500
-
 3 * 59500, SDS-PAGE Glycine max
3.2.2.16 180000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.16 5'-deoxy-5'-methylthioadenosine + H2O Glycine max
-
 ?
-
 ?
3.2.2.16 5'-methylthioadenosine + H2O Glycine max
-
 adenine + S-methyl-5-thio-D-ribose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.16 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.16 ammonium sulfate precipitation, acetone precipitation, Sephacryl S-200 gel filtration, DEAE-Sephacel gel filtration, and 5'-AMP-Sepharose column chromatography Glycine max
3.2.2.16 native enzyme 675.2fold from cotyledons by ammonium sulfate fractionation, acetone precipitation, gel filtration, anion exchange chromatography, and affinity chromatography Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.16 cotyledon
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.16 0.025
-
 crude extract, at pH 7.0 and 37°C Glycine max
3.2.2.16 16.88
-
 purified native enzyme, pH 7.0, 37°C Glycine max
3.2.2.16 16.88
-
 after 675.2fold purification, at pH 7.0 and 37°C Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.16 5'-deoxy-5'-methylthioadenosine + H2O
-
 Glycine max ?
-
 ?
3.2.2.16 5'-methylthioadenosine + H2O
-
 Glycine max adenine + S-methyl-5-thio-D-ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.16 homotrimer 3 * 59500, SDS-PAGE Glycine max
3.2.2.16 trimer 3 * 59500, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
3.2.2.16 5'-deoxy-5'-methylthioadenosine nucleosidase
-
 Glycine max
3.2.2.16 MTA nucleosidase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.16 37
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.16 7
-
 assay at Glycine max