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Literature summary extracted from

  • Neuhaus, F.C.
    Role of the omega loop in specificity determination in subsite 2 of the D-alanine:D-alanine (D-lactate) ligase from Leuconostoc mesenteroides: a molecular docking study (2011), J. Mol. Graph. Model., 30, 31-37.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.2.1 F261Y the mutant shows a complete loss of the ability to make D-alanyl-(R)-lactate Leuconostoc mesenteroides
6.3.2.4 F261Y mutation of the Omega loop, inactive mutant Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.2.1 D-alanine + (R)-lactate + ATP Leuconostoc mesenteroides
-
 D-alanyl-(R)-lactate + ADP + phosphate
-
 ?
6.3.2.4 ATP + 2 D-alanine Leuconostoc mesenteroides
-
 ADP + phosphate + D-alanyl-D-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.2.1 Leuconostoc mesenteroides
-
-
-
6.3.2.4 Leuconostoc mesenteroides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.2.1 D-alanine + (R)-lactate + ATP
-
 Leuconostoc mesenteroides D-alanyl-(R)-lactate + ADP + phosphate
-
 ?
6.3.2.4 ATP + 2 D-alanine
-
 Leuconostoc mesenteroides ADP + phosphate + D-alanyl-D-alanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.2.1 D-alanine:D-alanine (D-lactate) ligase (ADP)
-
 Leuconostoc mesenteroides
6.1.2.1 VanA
-
 Leuconostoc mesenteroides
6.1.2.1 VanB
-
 Leuconostoc mesenteroides
6.3.2.4 D-alanine:D-alanine (D-lactate) ligase (ADP)
-
 Leuconostoc mesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.2.1 ATP
-
 Leuconostoc mesenteroides
6.3.2.4 ATP
-
 Leuconostoc mesenteroides

General Information

EC Number General Information Comment Organism
6.3.2.4 additional information Phe261 is a key specificity determinant in the alpha-helical cap of the Omega-loop when folded into the closed conformation, molecular docking, overview. The hydroxyl of Tyr261 plays an instrumental role in determining non-productive docking orientations of dlactate, i.e. D-lactate-OH as an H-bond donor to the Tyr261-OH or D-lactate as an H-bond donor to the phosphoryl of the intermediate D-alanyl phosphate, and the D-lactate-COO- as an H-bond acceptor for the Tyr261-OH. Arg301 is required for the activation of the nucleophilic D-lactate for D-Ala-D-lactate formation. Ligand binding docking structure study, involving also the transition-state analogue, overview Leuconostoc mesenteroides