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Literature summary extracted from

  • Nam, J.S.; Yoon, J.H.; Lee, H.I.; Kim, S.W.; Ro, Y.T.
    Molecular cloning, purification, and characterization of a superoxide dismutase from a fast-growing Mycobacterium sp. strain JC1 DSM 3803 (2011), J. Microbiol., 49, 399-406.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene sodA, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree Mycobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2
-
 Mycobacterium sp.
1.15.1.1 additional information poor inhibition by KCN, no inhibition by EDTA and cuprizone Mycobacterium sp.
1.15.1.1 NaN3 slight inhibition Mycobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.15.1.1 cytosol
-
 Mycobacterium sp. 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ a cambialistic Fe/Mn-superoxide dismutase, 0.56 g-atom per mol of enzyme Mycobacterium sp.
1.15.1.1 Mn2+ a cambialistic Fe/Mn-superoxide dismutase, 1.12 g-atom per mol of enzyme Mycobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 22930
-
 2 * 23000, SDS-PAGE, 2 * 22930, sequence calculation Mycobacterium sp.
1.15.1.1 23000
-
 2 * 23000, SDS-PAGE, 2 * 22930, sequence calculation Mycobacterium sp.
1.15.1.1 48000
-
-
 Mycobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Mycobacterium sp. D0EAP3 gene sodA
-
1.15.1.1 Mycobacterium sp. DSM 3803 D0EAP3 gene sodA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 native enzyme 115fold by heat treatment at 60°C for 10 min, gel filtration, anion exchange and hydrophobic interaction chromatography Mycobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 culture condition:methanol-grown cell
-
 Mycobacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 2524
-
 purified native enzyme, pH 7.0, 25°C Mycobacterium sp.

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 23000, SDS-PAGE, 2 * 22930, sequence calculation Mycobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 SOD
-
 Mycobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 25
-
 assay at Mycobacterium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.15.1.1 10 60 activity range, profile overview Mycobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 4 50 purified enzyme, 60 min, completely stable Mycobacterium sp.
1.15.1.1 60
-
 purified enzyme, 60 min, loss of 20% activity Mycobacterium sp.
1.15.1.1 70
-
 purified enzyme, 60 min, inactivation Mycobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7
-
 assay at Mycobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 4 10 activity range, profile overview Mycobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 4 10 purified enzyme, 60 min, completely stable Mycobacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Mycobacterium sp. sequence calculation
-
 5.33