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Literature summary extracted from

  • Tinikul, R.; Thotsaporn, K.; Thaveekarn, W.; Jitrapakdee, S.; Chaiyen, P.
    The fusion Vibrio campbellii luciferase as a eukaryotic gene reporter (2012), J. Biotechnol., 162, 346-353.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.3 expressed in Escherichia coli BL21(DE3) and HEK-293T cells Vibrio campbellii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.3 77000
-
 2 * 77000, SDS-PAGE Vibrio campbellii
1.14.14.3 77800
-
 2 * 77800, calculated from amino acid sequence Vibrio campbellii
1.14.14.3 158000
-
 gel filtration Vibrio campbellii

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Vibrio campbellii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 RCHO + FMNH2 + O2
-
 Vibrio campbellii RCOOH + FMN + H2O + hv the enzyme is emitting blue-green light at 490 nm ?

Subunits

EC Number Subunits Comment Organism
1.14.14.3 homodimer 2 * 77000, SDS-PAGE Vibrio campbellii
1.14.14.3 homodimer 2 * 77800, calculated from amino acid sequence Vibrio campbellii

Synonyms

EC Number Synonyms Comment Organism
1.14.14.3 luciferase
-
 Vibrio campbellii
1.14.14.3 Lux
-
 Vibrio campbellii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.14.3 30 37 half-life of about 1020 min at 37°C. At 37°C, the native enzyme retains around 60% of the bioluminescence of the enzyme expressed at 30°C Vibrio campbellii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.3 7
-
-
 Vibrio campbellii