BRENDA - Enzyme Database show

Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1

Hirakawa, H.; Kamiya, N.; Kawarabayashi, Y.; Nagamune, T.; J. Biosci. Bioeng. 97, 202-206 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.1
expressed in Escherichia coli
Aeropyrum pernix
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.0004
-
NADH
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.001
-
NAD+
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.131
-
4-methoxyphenylacetone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.147
-
1-Hexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.147
-
2-decanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.215
-
2-nonanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.286
-
2-Octanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.333
-
benzaldehyde
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.396
-
1-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.596
-
1-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.694
-
tert-butyl acetoacetate
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.703
-
Cyclohexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.752
-
2-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.03
-
1-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.05
-
2-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.13
-
4-methoxybenzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.16
-
2-Heptanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.39
-
cyclohexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
2.44
-
2-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
5.01
-
2-Hexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
5.15
-
2-Pentanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
5.43
-
benzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
13.7
-
ethanol
pH 8.0, 60°C
Aeropyrum pernix
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
39570
-
4 * 39570, calculated from sequence
Aeropyrum pernix
1.1.1.1
40000
-
4 * 40000, SDS-PAGE
Aeropyrum pernix
1.1.1.1
160000
-
gel filtration
Aeropyrum pernix
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.1
Aeropyrum pernix
Q9Y9P9
-
-
1.1.1.1
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
Q9Y9P9
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.1
-
Aeropyrum pernix
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.1
1-butanol + NAD+
-
728012
Aeropyrum pernix
butanal + NADH + H+
-
-
-
?
1.1.1.1
1-butanol + NAD+
-
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
butanal + NADH + H+
-
-
-
?
1.1.1.1
1-hexanol + NAD+
-
728012
Aeropyrum pernix
1-hexanal + NADH + H+
-
-
-
?
1.1.1.1
1-pentanol + NAD+
-
728012
Aeropyrum pernix
1-pentanal + NADH + H+
-
-
-
?
1.1.1.1
1-propanol + NAD+
-
728012
Aeropyrum pernix
propanal + NADH + H+
-
-
-
?
1.1.1.1
1-propanol + NAD+
-
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
propanal + NADH + H+
-
-
-
?
1.1.1.1
2-butanol + NAD+
-
728012
Aeropyrum pernix
2-butanone + NADH + H+
-
-
-
?
1.1.1.1
2-butanol + NAD+
-
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
2-butanone + NADH + H+
-
-
-
?
1.1.1.1
2-decanone + NADH
-
728012
Aeropyrum pernix
(S)-2-decanol + NAD+ + H+
92% enantiomeric excess
-
-
?
1.1.1.1
2-heptanone + NADH
-
728012
Aeropyrum pernix
(S)-2-heptanol + NAD+ + H+
79% enantiomeric excess
-
-
?
1.1.1.1
2-hexanone + NADH
-
728012
Aeropyrum pernix
(S)-2-hexanol + NAD+ + H+
37% enantiomeric excess
-
-
?
1.1.1.1
2-nonanone + NADH
-
728012
Aeropyrum pernix
(S)-2-nonanol + NAD+ + H+
95% enantiomeric excess
-
-
?
1.1.1.1
2-octanone + NADH
-
728012
Aeropyrum pernix
(S)-2-octanol + NAD+ + H+
92% enantiomeric excess
-
-
?
1.1.1.1
2-pentanol + NAD+
-
728012
Aeropyrum pernix
2-pentanone + NADH + H+
-
-
-
?
1.1.1.1
2-pentanone + NADH
-
728012
Aeropyrum pernix
(S)-2-pentanol + NAD+ + H+
60% enantiomeric excess
-
-
?
1.1.1.1
2-propanol + NAD+
-
728012
Aeropyrum pernix
acetone + NADH + H+
-
-
-
?
1.1.1.1
2-propanol + NAD+
-
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
acetone + NADH + H+
-
-
-
?
1.1.1.1
4-methoxybenzyl alcohol + NAD+
-
728012
Aeropyrum pernix
4-methoxybenzaldehyde + NADH + H+
-
-
-
?
1.1.1.1
4-methoxyphenylacetone + NADH
-
728012
Aeropyrum pernix
(2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+
-
-
-
?
1.1.1.1
benzyl alcohol + NAD+
i.e. phenylmethanol
728012
Aeropyrum pernix
benzaldehyde + NADH + H+
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
728012
Aeropyrum pernix
cyclohexanone + NADH + H+
-
-
-
?
1.1.1.1
ethanol + NAD+
-
728012
Aeropyrum pernix
acetaldehyde + NADH + H+
-
-
-
?
1.1.1.1
additional information
the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone
728012
Aeropyrum pernix
?
-
-
-
-
1.1.1.1
additional information
the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
?
-
-
-
-
1.1.1.1
tert-butyl acetoacetate + NADH + H+
-
728012
Aeropyrum pernix
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.1
homotetramer
4 * 39570, calculated from sequence; 4 * 40000, SDS-PAGE
Aeropyrum pernix
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.1
95
-
catalytic activity increased up to 95°C
Aeropyrum pernix
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.1
70
-
30 min, no loss of activity
Aeropyrum pernix
1.1.1.1
80
-
30 min, about 20% loss of activity
Aeropyrum pernix
1.1.1.1
98
-
the enzyme maintains 24% of the original catalytic activity after incubation for 30 min
Aeropyrum pernix
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.071
-
4-methoxyphenylacetone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.072
-
tert-butyl acetoacetate
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.23
-
ethanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.24
-
2-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.26
-
1-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.37
-
1-Hexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.4
-
2-decanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.4
-
NAD+
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.41
-
1-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.41
-
NADH
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.45
-
1-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.48
-
2-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.52
-
Cyclohexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.6
-
2-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.6
-
4-methoxybenzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.7
1
2-nonanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.73
-
2-Heptanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.74
-
2-Octanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.77
-
2-Pentanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.02
-
benzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.08
-
2-Hexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.22
-
benzaldehyde
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.27
-
cyclohexanone
pH 8.0, 60°C
Aeropyrum pernix
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.1
8
-
reduction of 2-pentanone
Aeropyrum pernix
1.1.1.1
10.5
-
oxidation of 2-pentanol
Aeropyrum pernix
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.1
7
9.6
pH 7.0: about 70% of maximal activity, pH 9.6: about 50% of maximal activity, reduction of 2-pentanone
Aeropyrum pernix
1.1.1.1
9.6
11.5
pH 9.5: about 40% of maximal activity, pH 11.5: about 30% of maximal activity, oxidation of 2-pentanol
Aeropyrum pernix
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.1
expressed in Escherichia coli
Aeropyrum pernix
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.0004
-
NADH
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.001
-
NAD+
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.131
-
4-methoxyphenylacetone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.147
-
1-Hexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.147
-
2-decanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.215
-
2-nonanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.286
-
2-Octanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.333
-
benzaldehyde
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.396
-
1-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.596
-
1-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.694
-
tert-butyl acetoacetate
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.703
-
Cyclohexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.752
-
2-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.03
-
1-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.05
-
2-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.13
-
4-methoxybenzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.16
-
2-Heptanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.39
-
cyclohexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
2.44
-
2-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
5.01
-
2-Hexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
5.15
-
2-Pentanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
5.43
-
benzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
13.7
-
ethanol
pH 8.0, 60°C
Aeropyrum pernix
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.1
39570
-
4 * 39570, calculated from sequence
Aeropyrum pernix
1.1.1.1
40000
-
4 * 40000, SDS-PAGE
Aeropyrum pernix
1.1.1.1
160000
-
gel filtration
Aeropyrum pernix
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.1
-
Aeropyrum pernix
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.1
1-butanol + NAD+
-
728012
Aeropyrum pernix
butanal + NADH + H+
-
-
-
?
1.1.1.1
1-butanol + NAD+
-
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
butanal + NADH + H+
-
-
-
?
1.1.1.1
1-hexanol + NAD+
-
728012
Aeropyrum pernix
1-hexanal + NADH + H+
-
-
-
?
1.1.1.1
1-pentanol + NAD+
-
728012
Aeropyrum pernix
1-pentanal + NADH + H+
-
-
-
?
1.1.1.1
1-propanol + NAD+
-
728012
Aeropyrum pernix
propanal + NADH + H+
-
-
-
?
1.1.1.1
1-propanol + NAD+
-
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
propanal + NADH + H+
-
-
-
?
1.1.1.1
2-butanol + NAD+
-
728012
Aeropyrum pernix
2-butanone + NADH + H+
-
-
-
?
1.1.1.1
2-butanol + NAD+
-
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
2-butanone + NADH + H+
-
-
-
?
1.1.1.1
2-decanone + NADH
-
728012
Aeropyrum pernix
(S)-2-decanol + NAD+ + H+
92% enantiomeric excess
-
-
?
1.1.1.1
2-heptanone + NADH
-
728012
Aeropyrum pernix
(S)-2-heptanol + NAD+ + H+
79% enantiomeric excess
-
-
?
1.1.1.1
2-hexanone + NADH
-
728012
Aeropyrum pernix
(S)-2-hexanol + NAD+ + H+
37% enantiomeric excess
-
-
?
1.1.1.1
2-nonanone + NADH
-
728012
Aeropyrum pernix
(S)-2-nonanol + NAD+ + H+
95% enantiomeric excess
-
-
?
1.1.1.1
2-octanone + NADH
-
728012
Aeropyrum pernix
(S)-2-octanol + NAD+ + H+
92% enantiomeric excess
-
-
?
1.1.1.1
2-pentanol + NAD+
-
728012
Aeropyrum pernix
2-pentanone + NADH + H+
-
-
-
?
1.1.1.1
2-pentanone + NADH
-
728012
Aeropyrum pernix
(S)-2-pentanol + NAD+ + H+
60% enantiomeric excess
-
-
?
1.1.1.1
2-propanol + NAD+
-
728012
Aeropyrum pernix
acetone + NADH + H+
-
-
-
?
1.1.1.1
2-propanol + NAD+
-
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
acetone + NADH + H+
-
-
-
?
1.1.1.1
4-methoxybenzyl alcohol + NAD+
-
728012
Aeropyrum pernix
4-methoxybenzaldehyde + NADH + H+
-
-
-
?
1.1.1.1
4-methoxyphenylacetone + NADH
-
728012
Aeropyrum pernix
(2S)-1-(4-methoxyphenyl)propan-2-ol + NAD+ + H+
-
-
-
?
1.1.1.1
benzyl alcohol + NAD+
i.e. phenylmethanol
728012
Aeropyrum pernix
benzaldehyde + NADH + H+
-
-
-
?
1.1.1.1
cyclohexanol + NAD+
-
728012
Aeropyrum pernix
cyclohexanone + NADH + H+
-
-
-
?
1.1.1.1
ethanol + NAD+
-
728012
Aeropyrum pernix
acetaldehyde + NADH + H+
-
-
-
?
1.1.1.1
additional information
the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone
728012
Aeropyrum pernix
?
-
-
-
-
1.1.1.1
additional information
the enzyme shows broad substrate specificity and prefers aliphatic alcohols and ketones. There are no large differences in the reactivities between primary and secondary alcohols. The enzyme produces (S)-alcohols from the corresponding ketones. The values of the enantiomeric excess increase with the increase of chain length except for the reduction of 2-hexanone. The highest enantioselectivity is shown with the reduction of 2-nonanone
728012
Aeropyrum pernix ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1
?
-
-
-
-
1.1.1.1
tert-butyl acetoacetate + NADH + H+
-
728012
Aeropyrum pernix
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.1
homotetramer
4 * 39570, calculated from sequence; 4 * 40000, SDS-PAGE
Aeropyrum pernix
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.1
95
-
catalytic activity increased up to 95°C
Aeropyrum pernix
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.1
70
-
30 min, no loss of activity
Aeropyrum pernix
1.1.1.1
80
-
30 min, about 20% loss of activity
Aeropyrum pernix
1.1.1.1
98
-
the enzyme maintains 24% of the original catalytic activity after incubation for 30 min
Aeropyrum pernix
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.071
-
4-methoxyphenylacetone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.072
-
tert-butyl acetoacetate
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.23
-
ethanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.24
-
2-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.26
-
1-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.37
-
1-Hexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.4
-
2-decanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.4
-
NAD+
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.41
-
1-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.41
-
NADH
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.45
-
1-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.48
-
2-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.52
-
Cyclohexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.6
-
2-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.6
-
4-methoxybenzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.7
1
2-nonanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.73
-
2-Heptanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.74
-
2-Octanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.77
-
2-Pentanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.02
-
benzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.08
-
2-Hexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.22
-
benzaldehyde
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.27
-
cyclohexanone
pH 8.0, 60°C
Aeropyrum pernix
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.1
8
-
reduction of 2-pentanone
Aeropyrum pernix
1.1.1.1
10.5
-
oxidation of 2-pentanol
Aeropyrum pernix
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.1
7
9.6
pH 7.0: about 70% of maximal activity, pH 9.6: about 50% of maximal activity, reduction of 2-pentanone
Aeropyrum pernix
1.1.1.1
9.6
11.5
pH 9.5: about 40% of maximal activity, pH 11.5: about 30% of maximal activity, oxidation of 2-pentanol
Aeropyrum pernix
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.017
-
ethanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.097
-
2-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.1
-
tert-butyl acetoacetate
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.15
-
2-Pentanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.189
-
benzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.22
-
2-Hexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.25
-
1-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.46
-
2-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.53
-
4-methoxybenzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.54
-
4-methoxyphenylacetone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.62
-
2-Heptanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.69
-
1-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.73
-
Cyclohexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.79
-
2-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.91
-
cyclohexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.1
-
1-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
2.5
-
1-Hexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
2.6
-
2-Octanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
2.7
-
2-decanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
3.3
-
2-nonanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
3.66
-
benzaldehyde
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
380
-
NAD+
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1000
-
NADH
pH 8.0, 60°C
Aeropyrum pernix
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.1.1.1
0.017
-
ethanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.097
-
2-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.1
-
tert-butyl acetoacetate
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.15
-
2-Pentanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.189
-
benzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.22
-
2-Hexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.25
-
1-propanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.46
-
2-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.53
-
4-methoxybenzyl alcohol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.54
-
4-methoxyphenylacetone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.62
-
2-Heptanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.69
-
1-butanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.73
-
Cyclohexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.79
-
2-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
0.91
-
cyclohexanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1.1
-
1-Pentanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
2.5
-
1-Hexanol
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
2.6
-
2-Octanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
2.7
-
2-decanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
3.3
-
2-nonanone
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
3.66
-
benzaldehyde
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
380
-
NAD+
pH 8.0, 60°C
Aeropyrum pernix
1.1.1.1
1000
-
NADH
pH 8.0, 60°C
Aeropyrum pernix