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Literature summary extracted from

  • Toda, H.; Itoh, N.
    Isolation and characterization of styrene metabolism genes from styrene-assimilating soil bacteria Rhodococcus sp. ST-5 and ST-10 (2012), J. Biosci. Bioeng., 113, 12-19.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.11 expressed in Escherichia coli BL21(DE3) cells Rhodococcus sp.
5.3.99.7 gene styC, DNA and amino acid sequence determination and analysis, genetic organization, expression in Escherichia coli Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.11 46940
-
 x * 46940, calculated from amino acid sequence Rhodococcus sp.
1.14.14.11 47290
-
 x * 47290, calculated from amino acid sequence Rhodococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2 Rhodococcus sp.
-
 (S)-styrene oxide + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 Rhodococcus sp. ST-5
-
 (S)-styrene oxide + FAD + H2O
-
 ?
5.3.99.7 styrene oxide Rhodococcus sp.
-
 phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide Rhodococcus sp. ST-5
-
 phenylacetaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.11 Rhodococcus sp. G3XEX2
-
-
1.14.14.11 Rhodococcus sp. G3XEX5
-
-
1.14.14.11 Rhodococcus sp. ST-5 G3XEX5
-
-
5.3.99.7 no activity in Rhodococcus sp. ST-10
-
-
-
5.3.99.7 Rhodococcus sp. G3XEX7 gene styC, strain ST-5 has a gene cluster containing four open reading frames which encode styrene degradation enzymes
-
5.3.99.7 Rhodococcus sp. ST-5 G3XEX7 gene styC, strain ST-5 has a gene cluster containing four open reading frames which encode styrene degradation enzymes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2
-
 Rhodococcus sp. (S)-styrene oxide + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Rhodococcus sp. ST-5 (S)-styrene oxide + FAD + H2O
-
 ?
5.3.99.7 styrene oxide
-
 Rhodococcus sp. phenylacetaldehyde
-
 ?
5.3.99.7 styrene oxide
-
 Rhodococcus sp. ST-5 phenylacetaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.11 ? x * 46940, calculated from amino acid sequence Rhodococcus sp.
1.14.14.11 ? x * 47290, calculated from amino acid sequence Rhodococcus sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.14.11 SMO
-
 Rhodococcus sp.
1.14.14.11 StyA
-
 Rhodococcus sp.
5.3.99.7 styrene oxide isomerase
-
 Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.11 FADH2
-
 Rhodococcus sp.

General Information

EC Number General Information Comment Organism
5.3.99.7 metabolism the enzyme is involved in the styrene degradation pathway, overview Rhodococcus sp.
5.3.99.7 additional information in strain ST-10, which lacks a styrene oxide isomerase, styrene oxide is converted to phenylacetaldehyde by spontaneous chemical reaction. The strain accumulates styrene oxide as a metabolic intermediate. The produced phenylacetaldehyde is converted to phenylacetic acid in strain ST-10 as well as in strain ST-5 Rhodococcus sp.