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Literature summary extracted from
- The role of residue S139 of mandelate racemase: synergistic effect of S139 and E317 on transition state stabilization (2012), J. Biomol. Struct. Dyn., 30, 585-593.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.1.2.2 | S139A | site-directed mutagenesis, the mutation leads to a significant reduction of catalytic efficiency by about 45fold and 60fold in R to S and S to R directions | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.2.2 | (S)-mandelate | Pseudomonas putida | - |
(R)-mandelate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.2.2 | Pseudomonas putida | P11444 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.2.2 | (S)-mandelate | - |
Pseudomonas putida | (R)-mandelate | - |
? | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.1.2.2 | additional information | significance of Ser139 in mandelate racemization, the active site residue S139 forms a hydrogen bond with one carboxyl oxygen of the substrate mandelate, residues S139 and E317 of mandelate racemase have a synergistic effect on transition state stabilization, overview | Pseudomonas putida |
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Release 2023.1 (January 2023)
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