BRENDA - Enzyme Database

Substrate specificity of purified recombinant human beta-carotene 15,15'-oxygenase (BCO1)

Dela Sena, C.; Narayanasamy, S.; Riedl, K.M.; Curley, R.W.; Schwartz, S.J.; Harrison, E.H.; J. Biol. Chem. 288, 37094-37103 (2013)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.13.11.63
expression of BCO1 with a C-terminal polyhistidine tag in Escherichia coli strain BL21-Gold(DE3)
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.63
0.0172
-
beta-carotene
recombinant His6-tagged enzyme, pH and temperature not specified in the publication
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.63
63702
-
x * 63702, His-tagged enzyme, sequence calculation
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.13.11.63
beta-carotene + O2
Homo sapiens
-
2 all-trans-retinal
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.13.11.63
Homo sapiens
Q9HAY6
gene BCMO1; gene Bcmo1
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.13.11.63
recombinant BCO1 with a C-terminal polyhistidine tag from Escherichia coli strain BL21-Gold(DE3) by cobalt affinity chromatography
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.63
0.0022
-
purified recombinant His6-tagged enzyme, pH and temperature not specified in the publication
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.13.11.63
alpha-carotene + O2
-
727972
Homo sapiens
2 retinal
-
-
-
?
1.13.11.63
beta-apo-8'-carotenal + O2
-
727972
Homo sapiens
retinal + ?
-
-
-
?
1.13.11.63
beta-carotene + O2
-
727972
Homo sapiens
2 all-trans-retinal
-
-
-
?
1.13.11.63
beta-cryptoxanthin + O2
-
727972
Homo sapiens
2 retinal
-
-
-
?
1.13.11.63
lycopene + O2
-
727972
Homo sapiens
2 acycloretinal
-
-
-
?
1.13.11.63
additional information
shorter beta-apocarotenals, i.e. beta-apo-10-carotenal, beta-apo-12'-carotenal, and beta-apo-14'-carotenal, do not show Michaelis-Menten behavior under the conditions tested. No activity with lutein, zeaxanthin, and 9-cis-beta-carotene. Purified recombinant BCO1 cleaves beta-carotene solely at the central 15-15' bond
727972
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.63
?
x * 63702, His-tagged enzyme, sequence calculation
Homo sapiens
Synonyms
EC Number
Synonyms
Commentary
Organism
1.13.11.63
BCO1
-
Homo sapiens
1.13.11.63
beta-carotene 15,15'-oxygenase
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.63
expression of BCO1 with a C-terminal polyhistidine tag in Escherichia coli strain BL21-Gold(DE3)
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.63
0.0172
-
beta-carotene
recombinant His6-tagged enzyme, pH and temperature not specified in the publication
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.63
63702
-
x * 63702, His-tagged enzyme, sequence calculation
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.13.11.63
beta-carotene + O2
Homo sapiens
-
2 all-trans-retinal
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.63
recombinant BCO1 with a C-terminal polyhistidine tag from Escherichia coli strain BL21-Gold(DE3) by cobalt affinity chromatography
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.63
0.0022
-
purified recombinant His6-tagged enzyme, pH and temperature not specified in the publication
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.13.11.63
alpha-carotene + O2
-
727972
Homo sapiens
2 retinal
-
-
-
?
1.13.11.63
beta-apo-8'-carotenal + O2
-
727972
Homo sapiens
retinal + ?
-
-
-
?
1.13.11.63
beta-carotene + O2
-
727972
Homo sapiens
2 all-trans-retinal
-
-
-
?
1.13.11.63
beta-cryptoxanthin + O2
-
727972
Homo sapiens
2 retinal
-
-
-
?
1.13.11.63
lycopene + O2
-
727972
Homo sapiens
2 acycloretinal
-
-
-
?
1.13.11.63
additional information
shorter beta-apocarotenals, i.e. beta-apo-10-carotenal, beta-apo-12'-carotenal, and beta-apo-14'-carotenal, do not show Michaelis-Menten behavior under the conditions tested. No activity with lutein, zeaxanthin, and 9-cis-beta-carotene. Purified recombinant BCO1 cleaves beta-carotene solely at the central 15-15' bond
727972
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.63
?
x * 63702, His-tagged enzyme, sequence calculation
Homo sapiens