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Literature summary extracted from

  • Takusagawa, F.
    Microsomal prostaglandin E synthase type 2 (mPGES2) is a glutathione-dependent heme protein, and dithiothreitol dissociates the bound heme to produce active prostaglandin E2 synthase in vitro (2013), J. Biol. Chem., 288, 10166-10175.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.99.3 dithiothreitol leads to dissociation of the bound heme which is hindered by imidazole Macaca fascicularis
5.3.99.3 GSH mPGES2 has a GSH-binding motif, the GSH molecule binds at the predicted amino acid residues 148VPxL to 164DSxxI. GSH is essential for heme binding to isozyme mPGES2, structures of GSH-heme complex-binding cavity in isozyme mPGES2, overview Macaca fascicularis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.99.3 heme heme is bound to the enzyme subunits and alters the enzyme catalytic specificity, overview. Heme is attached to the bound GSH by an iron-sulfur bond, but heme cannot bind to mPGES2 in the absence of GSH, other sulfhydryl compounds, such as DTT and 2-mercaptoethanol, are unable to promote heme binding to isozyme mPGES2. Bound heme is oxidized by H2O2, but it does not dissociate from mPGES2 Macaca fascicularis
5.3.99.3 imidazole imidazole stacks on the bound heme and inhibits heme oxidation by H2O2, heme dissociation by DTT, and catalytic activity Macaca fascicularis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 membrane membrane-associated prostaglandin E synthase-2 Macaca fascicularis 16020
-
5.3.99.3 microsome
-
 Macaca fascicularis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.3 (5Z,13E,15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate Macaca fascicularis reaction of heme-free isozyme mPGES2 (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Macaca fascicularis Q9N0A4 isozyme mPGES2
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.99.3 liver
-
 Macaca fascicularis
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5.3.99.3 additional information abundant tissue distribution of isozyme mPGES2 Macaca fascicularis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 (5Z,13E,15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate reaction of heme-free isozyme mPGES2 Macaca fascicularis (5Z,13E,15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
-
 ?
5.3.99.3 additional information heme-free isozyme mPGES2 and heme-bound isozyme mPGES2 catalyze isomerization of prostaglandin H2 to prostaglandin E2 and conversion of prostaglandin H2 to to (12S)-hydroxy-(5Z,8E,10E)-heptadecatrienoic acid and malondialdehyde, respectively Macaca fascicularis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.3 membrane-associated prostaglandin E synthase-2
-
 Macaca fascicularis
5.3.99.3 MPGES2
-
 Macaca fascicularis
5.3.99.3 prostaglandin E2 synthase
-
 Macaca fascicularis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.99.3 4
-
 assay at Macaca fascicularis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.99.3 7.2
-
 assay at Macaca fascicularis

Expression

EC Number Organism Comment Expression
5.3.99.3 Macaca fascicularis iron does not induce the enzyme expression additional information