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Literature summary extracted from

  • Nakamura, A.; Fujihashi, M.; Aono, R.; Sato, T.; Nishiba, Y.; Yoshida, S.; Yano, A.; Atomi, H.; Imanaka, T.; Miki, K.
    Dynamic, ligand-dependent conformational change triggers reaction of ribose-1,5-bisphosphate isomerase from Thermococcus kodakarensis KOD1 (2012), J. Biol. Chem., 287, 20784-20796.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.29 expression in Escherichia coli Thermococcus kodakarensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.29 sitting drop vapor diffusion method at 20°C. Crystal structure of unliganded Tk-R15Pi is solved by means of the single-wavelength anomalous dispersion method using SeMet-substituted enzyme and refined at 2.5 A resolution Thermococcus kodakarensis

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.29 C133A inactive mutant enzyme Thermococcus kodakarensis
5.3.1.29 C133S inactive mutant enzyme Thermococcus kodakarensis
5.3.1.29 D202N inactive mutant enzyme Thermococcus kodakarensis
5.3.1.29 R227R mutant enzyme exhibits a decrease in molecular size of the enzyme and significantly decreases the enzymatic activity Thermococcus kodakarensis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.29 Thermococcus kodakarensis Q5JFM9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.29
-
 Thermococcus kodakarensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.29 29.3
-
 pH 8.0, 85°C, wild-type enzyme Thermococcus kodakarensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.29 alpha-D-ribose 1,5-bisphosphate the enzyme is specific for the alpha-anomer of D-ribose 1,5-bisphosphate Thermococcus kodakarensis D-ribulose 1,5-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.29 hexamer
-
 Thermococcus kodakarensis

Synonyms

EC Number Synonyms Comment Organism
5.3.1.29 R15Pi
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.29 85
-
 assay at Thermococcus kodakarensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.29 8
-
 assay at Thermococcus kodakarensis