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Literature summary extracted from

  • Nayak, B.; Kondeti, V.K.; Xie, P.; Lin, S.; Viswakarma, N.; Raparia, K.; Kanwar, Y.S.
    Transcriptional and post-translational modulation of myo-inositol oxygenase by high glucose and related pathobiological stresses (2011), J. Biol. Chem., 286, 27594-27611.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.99.1 expressed in Escherichia coli Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.13.99.1 S24A/S205A/S218A/T29A/T69A mutated putative PKA phosphorylation sites: mutant is also phosphorylated but to a minor degree Mus musculus
1.13.99.1 S24A/S205A/S218A/T29A/T69A/S64A/T40A/T49A/T69A/T253A mutated putative PKA and PKC phosphorylation sites: mutant is also phosphorylated but to a minor degree Mus musculus
1.13.99.1 S64A/T40A/T49A/T69A/T253A mutated putative PKC phosphorylation sites: mutant is also phosphorylated but to a minor degree Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.13.99.1 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.13.99.1 phosphoprotein mutants with substituted phosphorylation sites have a minimal increase in activity. Treatment of cells with PKC, PKA, and PDK1 kinase activators increased activity, whereas inhibitors reduced it. Protein kinases A, C, and PDK1 are capable of phosphorylating mouse in both prokaryotic and eukaryotic systems. Using deletion constructs it is shown that the N-terminus contains the critical phosphorylation sites that are highly relevant to the functionality of the protein Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.99.1 kidney
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.99.1 myo-inositol + O2
-
 Mus musculus D-glucuronic acid + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.99.1 Myo-inositol oxygenase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.99.1 30
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.99.1 8
-
 assay at Mus musculus

Expression

EC Number Organism Comment Expression
1.13.99.1 Mus musculus antioxidants N-acetylcysteine, beta-naphthoflavone, and tertiary butyl hydroquinone reduces MIOX expression down
1.13.99.1 Mus musculus high glucose levels, exposure of cells to oxidants H2O2 and methylglyoxal up-regulates MIOX expression up