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Literature summary extracted from

  • Tripp, B.C.; Bell, C.B.; Cruz, F.; Krebs, C.; Ferry, J.G.
    A role for iron in an ancient carbonic anhydrase (2004), J. Biol. Chem., 279, 6683-6687.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 expression in Escherichia coli Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 additional information
-
 additional information kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Fe2+ anaerobic reconstitution of apoenzyme with Fe2+, which yields an enzyme with an effective kcat that exceeds that for the Zn2+-reconstituted enzyme. The Fe2+-reconstituted enzyme contains high spin Fe2+ that, when oxidized to Fe3+, inactivates the enzyme. Reconstitution with Fe3+ is unsuccessful Methanosarcina thermophila
4.2.1.1 Fe2+ the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme. The Fe2+-reconstituted enzyme contains high spin Fe2+ that, when oxidized to Fe3+, inactivates the enzyme. Reconstitution with Fe3+ is unsuccessful Methanosarcina thermophila
4.2.1.1 additional information reconstitution of the apoenzyme with Cu2+, Mn2+, Ni2+, or Cd2+ yields enzymes with effective kcat values that are 10% or less than the value for the Zn2+-reconstituted apoenzyme Methanosarcina thermophila
4.2.1.1 Zn2+ the enzyme contains contains catalytic zinc Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Methanosarcina thermophila
-
-
-
4.2.1.1 Methanosarcina thermophila P40881
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 the recombinant enzyme is purified anaerobically Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Methanosarcina thermophila H2CO3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 Cam
-
 Methanosarcina thermophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 additional information
-
 additional information kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ Methanosarcina thermophila
4.2.1.1 additional information
-
 additional information the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme Methanosarcina thermophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.1 additional information
-
 additional information kinetic constants of apoenzyme reconstituted with Zn2+, Co2+, Cu2+, Mn2+, Ni2+, Cd2+, or Fe2+ Methanosarcina thermophila
4.2.1.1 additional information
-
 additional information the Fe2+-reconstituted enzyme produced in Escherichia coli and purified anaerobically contains iron with effective kcat and kcat/Km values exceeding the values for Zn2+-reconstituted enzyme Methanosarcina thermophila