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Literature summary extracted from

  • Pierrel, F.; Douki, T.; Fontecave, M.; Atta, M.
    MiaB protein is a bifunctional radical-S-adenosylmethionine enzyme involved in thiolation and methylation of tRNA (2004), J. Biol. Chem., 279, 47555-47563.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.3 Thermotoga maritima Q9WZC1
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2.8.4.3 Thermotoga maritima DSM 3109 Q9WZC1
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.3 additional information MiaB protein is a bifunctional system, involved in both thiolation and methylation of N6-dimethylallyladenine. In this process, one molecule of S-adenosyl-L-methionine is converted to 5'-deoxyadenosine, probably through reductive cleavage and intermediate formation of a 5'-deoxyadenosyl radical as observed in other radical-S-adenosyl-L-methionine enzymes, and a second molecule of S-adenosyl-L-methionine is used as a methyl donor Thermotoga maritima ?
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 ?
2.8.4.3 additional information MiaB protein is a bifunctional system, involved in both thiolation and methylation of N6-dimethylallyladenine. In this process, one molecule of S-adenosyl-L-methionine is converted to 5'-deoxyadenosine, probably through reductive cleavage and intermediate formation of a 5'-deoxyadenosyl radical as observed in other radical-S-adenosyl-L-methionine enzymes, and a second molecule of S-adenosyl-L-methionine is used as a methyl donor Thermotoga maritima DSM 3109 ?
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 ?
2.8.4.3 N6-dimethylallyladenine in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine substrate is a synthetic N6-dimethylallyladenine-containing RNA corresponding to the anticodon stem loop of tRNAPhe Thermotoga maritima 2-methylthio-N6-dimethylallyladenine in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?
2.8.4.3 N6-dimethylallyladenine in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine substrate is a synthetic N6-dimethylallyladenine-containing RNA corresponding to the anticodon stem loop of tRNAPhe Thermotoga maritima DSM 3109 2-methylthio-N6-dimethylallyladenine in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine overall reaction ?
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine
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 Thermotoga maritima 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine
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 ?
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine
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 Thermotoga maritima DSM 3109 2-methylthio-N6-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine
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 ?
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine
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 Thermotoga maritima 2-thio-N6-dimethylallyladenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine
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 ?
2.8.4.3 N6-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine
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 Thermotoga maritima DSM 3109 2-thio-N6-dimethylallyladenine37 in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine
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 ?
2.8.4.3 S-adenosyl-L-methionine + 2-thio-N6-dimethylallyladenine37 in tRNA
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 Thermotoga maritima S-adenosyl-L-homocysteine + 2-methylthio-N6-dimethylallyladenine37 in tRNA
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 ?
2.8.4.3 S-adenosyl-L-methionine + 2-thio-N6-dimethylallyladenine37 in tRNA
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 Thermotoga maritima DSM 3109 S-adenosyl-L-homocysteine + 2-methylthio-N6-dimethylallyladenine37 in tRNA
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 ?