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Literature summary extracted from

  • Kneidinger, B.; ORiordan, K.; Li, J.; Brisson, J.; Lee, J.; Lam, J.
    Three highly conserved proteins catalyze the conversion of UDP-N-acetyl-D-glucosamine to precursors for the biosynthesis of O antigen in Pseudomonas aeruginosa O11 and capsule in Staphylococcus aureus type 5: Implications for the UDP-N-acetyl-L-fucosamine (2003), J. Biol. Chem., 278, 3615-3627.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.367 expression in Escherichia coli Pseudomonas aeruginosa
1.1.1.367 expression in Escherichia coli Staphylococcus aureus
5.1.3.28 expressed in Escherichia coli Staphylococcus aureus
5.1.3.28 expressed in Escherichia coli Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.367 43600
-
x * 43600, SDS-PAGE Pseudomonas aeruginosa
1.1.1.367 43600
-
x * 43600, SDS-PAGE Staphylococcus aureus
5.1.3.28 43900
-
x * 43900, calculated from amino acid sequence Staphylococcus aureus
5.1.3.28 43900
-
x * 43900, calculated from amino acid sequence Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.367 Pseudomonas aeruginosa
-
-
-
1.1.1.367 Pseudomonas aeruginosa O11
-
-
-
1.1.1.367 Staphylococcus aureus P95700
-
-
1.1.1.367 Staphylococcus aureus Newman P95700
-
-
5.1.3.28 Pseudomonas aeruginosa
-
-
-
5.1.3.28 Pseudomonas aeruginosa O11
-
-
-
5.1.3.28 Staphylococcus aureus
-
-
-
5.1.3.28 Staphylococcus aureus Newman
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.28 nickel chelating column chromatography Staphylococcus aureus
5.1.3.28 nickel chelating column chromatography Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.1.1.367 -20°C, 40% glycerol, stable for several weeks Pseudomonas aeruginosa
1.1.1.367 -20°C, 40% glycerol, stable for several weeks Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.367 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + NADPH + H+
-
Staphylococcus aureus UDP-2-acetamido-2,6-dideoxy-beta-L-talose + NADP+ + H+ enzyme Cap5F is responsible for the reduction at C4 of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose ?
1.1.1.367 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + NADPH + H+
-
Pseudomonas aeruginosa UDP-2-acetamido-2,6-dideoxy-beta-L-talose + NADP+ + H+ enzyme WbjC is responsible for the reduction at C4 of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose ?
1.1.1.367 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + NADPH + H+
-
Staphylococcus aureus Newman UDP-2-acetamido-2,6-dideoxy-beta-L-talose + NADP+ + H+ enzyme Cap5F is responsible for the reduction at C4 of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose ?
1.1.1.367 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + NADPH + H+
-
Pseudomonas aeruginosa O11 UDP-2-acetamido-2,6-dideoxy-beta-L-talose + NADP+ + H+ enzyme WbjC is responsible for the reduction at C4 of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose ?
5.1.3.28 additional information no activity with UDP-D-GlcNAc Staphylococcus aureus ?
-
?
5.1.3.28 additional information no activity with UDP-D-GlcNAc Pseudomonas aeruginosa ?
-
?
5.1.3.28 additional information no activity with UDP-D-GlcNAc Staphylococcus aureus Newman ?
-
?
5.1.3.28 additional information no activity with UDP-D-GlcNAc Pseudomonas aeruginosa O11 ?
-
?
5.1.3.28 UDP-2-acetamido-2,6-dideoxy-beta-L-talose
-
Staphylococcus aureus UDP-N-acetyl-beta-L-fucosamine
-
?
5.1.3.28 UDP-2-acetamido-2,6-dideoxy-beta-L-talose
-
Pseudomonas aeruginosa UDP-N-acetyl-beta-L-fucosamine
-
?
5.1.3.28 UDP-2-acetamido-2,6-dideoxy-beta-L-talose
-
Staphylococcus aureus Newman UDP-N-acetyl-beta-L-fucosamine
-
?
5.1.3.28 UDP-2-acetamido-2,6-dideoxy-beta-L-talose
-
Pseudomonas aeruginosa O11 UDP-N-acetyl-beta-L-fucosamine
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.367 ? x * 43600, SDS-PAGE Pseudomonas aeruginosa
1.1.1.367 ? x * 43600, SDS-PAGE Staphylococcus aureus
5.1.3.28 ? x * 43900, calculated from amino acid sequence Staphylococcus aureus
5.1.3.28 ? x * 43900, calculated from amino acid sequence Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.367 Cap5F
-
Staphylococcus aureus
1.1.1.367 O11 O-antigen biosynthesis associated epimerase
-
Pseudomonas aeruginosa
1.1.1.367 WbjC
-
Pseudomonas aeruginosa
5.1.3.28 Cap5G
-
Staphylococcus aureus
5.1.3.28 WbjD
-
Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
1.1.1.367 physiological function involved in biosynthesis of N-acetyl-L-fucosamine. Enzyme Cap5E catalyzes 4,6-dehydration of UDP-N-acetyl-Dglucosamine and 3- and 5-epimerization to yield a mixture of three keto-deoxy-sugars. UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose is subsequently reduced at C4 to UDP-2-acetamido-2,6-dideoxy-L-talose by Cap5F. Incubation of UDP-2-acetamido-2,6-dideoxy-L-talose with Cap5G results in 2-epimerization to N-acetyl-L-fucosamine Staphylococcus aureus
1.1.1.367 physiological function involved in biosynthesis of N-acetyl-L-fucosamine. Enzyme WbjB catalyzes 4,6-dehydration of UDP-N-acetyl-Dglucosamine and 3- and 5-epimerization to yield a mixture of three keto-deoxy-sugars. UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose is subsequently reduced at C4 to UDP-2-acetamido-2,6-dideoxy-L-talose by WbjC. Incubation of UDP-2-acetamido-2,6-dideoxy-L-talose with WbjD results in 2-epimerization to N-acetyl-L-fucosamine Pseudomonas aeruginosa