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Literature summary extracted from

  • Aceti, D.J.; Ferry, J.G.
    Purification and characterization of acetate kinase from acetate-grown Methanosarcina thermophila. Evidence for regulation of synthesis (1988), J. Biol. Chem., 263, 15444-15448.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.2.1 the enzyme is unaffected by repeated freezing and thawing Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 KCl activity linearly decreases from 100% (at 0 mM added KCl) to 71% at 500 mM added KCl Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 2.8
-
 ATP 37°C, pH is not specified in the publication Methanosarcina thermophila
2.7.2.1 22
-
 acetate 37°C, pH is not specified in the publication Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Ca2+ the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity Methanosarcina thermophila
2.7.2.1 Co2+ the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity Methanosarcina thermophila
2.7.2.1 Mg2+ the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity Methanosarcina thermophila
2.7.2.1 Mn2+ the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). The maximum rate is obtained with Mn2+ Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 53000
-
 2 * 53000, denaturing gel electrophoresis Methanosarcina thermophila
2.7.2.1 87000
-
 non-denaturing, gradient gel electrophoresis Methanosarcina thermophila
2.7.2.1 94000
-
 gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Methanosarcina thermophila the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 ADP + acetyl phosphate
-
 ?
2.7.2.1 ATP + acetate Methanosarcina thermophila TM-1 the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 ADP + acetyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methanosarcina thermophila P38502
-
-
2.7.2.1 Methanosarcina thermophila TM-1 P38502
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.2.1 the enzyme is stable to oxygen Methanosarcina thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Methanosarcina thermophila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.1 culture condition:acetate-grown cell
-
 Methanosarcina thermophila
-

Storage Stability

EC Number Storage Stability Organism
2.7.2.1 frozen in liquid nitrogen, the enzyme is stable for at least months Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 Methanosarcina thermophila ADP + acetyl phosphate
-
 ?
2.7.2.1 ATP + acetate activity in the reverse direction is 2.6fold greater than in the forward direction (acetate kinase activity) Methanosarcina thermophila ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 Methanosarcina thermophila TM-1 ADP + acetyl phosphate
-
 ?
2.7.2.1 ATP + acetate activity in the reverse direction is 2.6fold greater than in the forward direction (acetate kinase activity) Methanosarcina thermophila TM-1 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + propionate the enzyme phosphorylates propionate at 60% of the rate with acetate Methanosarcina thermophila ADP + propionyl phosphate
-
 ?
2.7.2.1 CTP + acetate activity with TP is 53% of the activity with ATP Methanosarcina thermophila CDP + acetyl phosphate
-
 ?
2.7.2.1 GTP + acetate activity with TP is 79% of the activity with ATP Methanosarcina thermophila GDP + acetyl phosphate
-
 ?
2.7.2.1 GTP + acetate activity with TP is 79% of the activity with ATP Methanosarcina thermophila TM-1 GDP + acetyl phosphate
-
 ?
2.7.2.1 ITP + acetate activity with TP is 83% of the activity with ATP Methanosarcina thermophila IDP + acetyl phosphate
-
 ?
2.7.2.1 ITP + acetate activity with TP is 83% of the activity with ATP Methanosarcina thermophila TM-1 IDP + acetyl phosphate
-
 ?
2.7.2.1 additional information the enzyme is unable to use formate Methanosarcina thermophila ?
-
 ?
2.7.2.1 additional information the enzyme is unable to use formate Methanosarcina thermophila TM-1 ?
-
 ?
2.7.2.1 TTP + acetate activity with TP is 106% of the activity with ATP Methanosarcina thermophila TDP + acetyl phosphate
-
 ?
2.7.2.1 UTP + acetate activity with TP is 80% of the activity with ATP Methanosarcina thermophila UDP + acetyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.1 homodimer 2 * 53000, denaturing gel electrophoresis Methanosarcina thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.1 37
-
 assay at Methanosarcina thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.1 70
-
 stable for 15 min, rapidly inactivated at higher temperature Methanosarcina thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7 7.4 acetate kinase activity Methanosarcina thermophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.1 6 8.5 pH 6.0: about 75% of maximal activity, pH 8.5: about 65% of maximal activity, acetate kinase activity Methanosarcina thermophila

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.1 Methanosarcina thermophila isoelectric focusing
-
 4.7

General Information

EC Number General Information Comment Organism
2.7.2.1 physiological function the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 Methanosarcina thermophila