EC Number | General Stability | Organism |
---|---|---|
2.7.2.1 | the enzyme is unaffected by repeated freezing and thawing | Methanosarcina thermophila |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.1 | KCl | activity linearly decreases from 100% (at 0 mM added KCl) to 71% at 500 mM added KCl | Methanosarcina thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 2.8 | - |
ATP | 37°C, pH is not specified in the publication | Methanosarcina thermophila | |
2.7.2.1 | 22 | - |
acetate | 37°C, pH is not specified in the publication | Methanosarcina thermophila |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.1 | Ca2+ | the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity | Methanosarcina thermophila | |
2.7.2.1 | Co2+ | the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity | Methanosarcina thermophila | |
2.7.2.1 | Mg2+ | the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). Cu2+, Ni2+, or Zn2+ resulted in no significanta activity | Methanosarcina thermophila | |
2.7.2.1 | Mn2+ | the enzyme requires a divalent cations for activity (Mn2+, Mg2+ Co2+ or Ca2+). The maximum rate is obtained with Mn2+ | Methanosarcina thermophila |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 53000 | - |
2 * 53000, denaturing gel electrophoresis | Methanosarcina thermophila |
2.7.2.1 | 87000 | - |
non-denaturing, gradient gel electrophoresis | Methanosarcina thermophila |
2.7.2.1 | 94000 | - |
gel filtration | Methanosarcina thermophila |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | Methanosarcina thermophila | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | ADP + acetyl phosphate | - |
? | |
2.7.2.1 | ATP + acetate | Methanosarcina thermophila TM-1 | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | ADP + acetyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.1 | Methanosarcina thermophila | P38502 | - |
- |
2.7.2.1 | Methanosarcina thermophila TM-1 | P38502 | - |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
2.7.2.1 | the enzyme is stable to oxygen | Methanosarcina thermophila |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.1 | - |
Methanosarcina thermophila |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.2.1 | culture condition:acetate-grown cell | - |
Methanosarcina thermophila | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.2.1 | frozen in liquid nitrogen, the enzyme is stable for at least months | Methanosarcina thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | Methanosarcina thermophila | ADP + acetyl phosphate | - |
? | |
2.7.2.1 | ATP + acetate | activity in the reverse direction is 2.6fold greater than in the forward direction (acetate kinase activity) | Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
2.7.2.1 | ATP + acetate | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | Methanosarcina thermophila TM-1 | ADP + acetyl phosphate | - |
? | |
2.7.2.1 | ATP + acetate | activity in the reverse direction is 2.6fold greater than in the forward direction (acetate kinase activity) | Methanosarcina thermophila TM-1 | ADP + acetyl phosphate | - |
r | |
2.7.2.1 | ATP + propionate | the enzyme phosphorylates propionate at 60% of the rate with acetate | Methanosarcina thermophila | ADP + propionyl phosphate | - |
? | |
2.7.2.1 | CTP + acetate | activity with TP is 53% of the activity with ATP | Methanosarcina thermophila | CDP + acetyl phosphate | - |
? | |
2.7.2.1 | GTP + acetate | activity with TP is 79% of the activity with ATP | Methanosarcina thermophila | GDP + acetyl phosphate | - |
? | |
2.7.2.1 | GTP + acetate | activity with TP is 79% of the activity with ATP | Methanosarcina thermophila TM-1 | GDP + acetyl phosphate | - |
? | |
2.7.2.1 | ITP + acetate | activity with TP is 83% of the activity with ATP | Methanosarcina thermophila | IDP + acetyl phosphate | - |
? | |
2.7.2.1 | ITP + acetate | activity with TP is 83% of the activity with ATP | Methanosarcina thermophila TM-1 | IDP + acetyl phosphate | - |
? | |
2.7.2.1 | additional information | the enzyme is unable to use formate | Methanosarcina thermophila | ? | - |
? | |
2.7.2.1 | additional information | the enzyme is unable to use formate | Methanosarcina thermophila TM-1 | ? | - |
? | |
2.7.2.1 | TTP + acetate | activity with TP is 106% of the activity with ATP | Methanosarcina thermophila | TDP + acetyl phosphate | - |
? | |
2.7.2.1 | UTP + acetate | activity with TP is 80% of the activity with ATP | Methanosarcina thermophila | UDP + acetyl phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.2.1 | homodimer | 2 * 53000, denaturing gel electrophoresis | Methanosarcina thermophila |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 37 | - |
assay at | Methanosarcina thermophila |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 70 | - |
stable for 15 min, rapidly inactivated at higher temperature | Methanosarcina thermophila |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 7 | 7.4 | acetate kinase activity | Methanosarcina thermophila |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 6 | 8.5 | pH 6.0: about 75% of maximal activity, pH 8.5: about 65% of maximal activity, acetate kinase activity | Methanosarcina thermophila |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.2.1 | Methanosarcina thermophila | isoelectric focusing | - |
4.7 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.2.1 | physiological function | the enzyme is proposed to function in the initial activation of acetate for conversion to methane and CO2 | Methanosarcina thermophila |