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Literature summary extracted from

  • Vannice, J.C.; Skaff, D.A.; Keightley, A.; Addo, J.K.; Wyckoff, G.J.; Miziorko, H.M.
    Identification in Haloferax volcanii of phosphomevalonate decarboxylase and isopentenyl phosphate kinase as catalysts of the terminal enzyme reactions in an archaeal alternate mevalonate pathway (2014), J. Bacteriol., 196, 1055-1063.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.26 expression of an N-terminally His-tagged enzyme form in Haloferax volcanii H1209 Haloferax volcanii
4.1.1.99 expression in Haloferax volcanii H1209 Haloferax volcanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.33 6-fluoromevalonate diphosphate negligible inhibition Haloferax volcanii
4.1.1.33 6-fluoromevalonate monophosphate strong inhibition Haloferax volcanii
4.1.1.99 6-fluoromevalonate 5'-phosphate negligible inhibition by 6-fluoromevalonate diphosphate (a potent inhibitor of the classical mevalonate pathway), reinforcing its selectivity for monophosphorylated ligands Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.26 0.077
-
 isopentenyl phosphate pH 7.5, 30°C Haloferax volcanii
2.7.4.26 0.29
-
 ATP pH 7.5, 30°C Haloferax volcanii
4.1.1.99 0.075
-
 mevalonate 5-phosphate pH 7.5, 30°C Haloferax volcanii
4.1.1.99 0.289
-
 ATP pH 7.5, 30°C Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.99 KCl activity is not strongly dependent on KCl over a 0 to 4 M range Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.26 26417
-
 x * 26417, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii
4.1.1.99 37328
-
 x * 37328, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.26 ATP + isopentenyl phosphate Haloferax volcanii the enzyme is involved in the mevalonate pathway in archaea ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate Haloferax volcanii DSM 3757 the enzyme is involved in the mevalonate pathway in archaea ADP + isopentenyl diphosphate
-
 ?
4.1.1.33 ATP + (R)-5-diphosphomevalonate Haloferax volcanii
-
 ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?
4.1.1.99 ATP + mevalonate 5-phosphate Haloferax volcanii the enzyme is required for the alternate mevalonate pathway in an archaea ADP + phosphate + isopentenyl phosphate + CO2
-
 ?
4.1.1.99 ATP + mevalonate 5-phosphate Haloferax volcanii DSM 3757 the enzyme is required for the alternate mevalonate pathway in an archaea ADP + phosphate + isopentenyl phosphate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.26 Haloferax volcanii D4GWT7
-
-
2.7.4.26 Haloferax volcanii DSM 3757 D4GWT7
-
-
4.1.1.33 Haloferax volcanii
-
-
-
4.1.1.99 Haloferax volcanii D4GXZ3
-
-
4.1.1.99 Haloferax volcanii DSM 3757 D4GXZ3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.26
-
 Haloferax volcanii
4.1.1.99
-
 Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.26 ATP + isopentenyl phosphate
-
 Haloferax volcanii ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate the enzyme is involved in the mevalonate pathway in archaea Haloferax volcanii ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate
-
 Haloferax volcanii DSM 3757 ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate the enzyme is involved in the mevalonate pathway in archaea Haloferax volcanii DSM 3757 ADP + isopentenyl diphosphate
-
 ?
4.1.1.33 ATP + (R)-5-diphosphomevalonate
-
 Haloferax volcanii ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?
4.1.1.99 ATP + mevalonate 5-phosphate the enzyme is required for the alternate mevalonate pathway in an archaea Haloferax volcanii ADP + phosphate + isopentenyl phosphate + CO2
-
 ?
4.1.1.99 ATP + mevalonate 5-phosphate very weak activity with mevalonate and even less with mevalonate 5-diphosphate Haloferax volcanii ADP + phosphate + isopentenyl phosphate + CO2
-
 ?
4.1.1.99 ATP + mevalonate 5-phosphate the enzyme is required for the alternate mevalonate pathway in an archaea Haloferax volcanii DSM 3757 ADP + phosphate + isopentenyl phosphate + CO2
-
 ?
4.1.1.99 ATP + mevalonate 5-phosphate very weak activity with mevalonate and even less with mevalonate 5-diphosphate Haloferax volcanii DSM 3757 ADP + phosphate + isopentenyl phosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.26 ? x * 26417, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii
4.1.1.99 ? x * 37328, His-tagged enzyme, matrix-assisted laser desorption ionization-time of flight mass spectrometry Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
2.7.4.26 HvIpk
-
 Haloferax volcanii
2.7.4.26 HVO_2762
-
 Haloferax volcanii
4.1.1.33 phosphomevalonate decarboxylase
-
 Haloferax volcanii
4.1.1.33 PMD
-
 Haloferax volcanii
4.1.1.99 HVO_1412
-
 Haloferax volcanii
4.1.1.99 PMD
-
 Haloferax volcanii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.26 16.3
-
 ATP pH 7.5, 30°C Haloferax volcanii
2.7.4.26 16.3
-
 isopentenyl phosphate pH 7.5, 30°C Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.26 7.5
-
 assay at Haloferax volcanii
4.1.1.99 7.5
-
-
 Haloferax volcanii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.26 Haloferax volcanii calculated from sequence
-
 5.06

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.1.99 0.016
-
 pH 7.5, 30°C Haloferax volcanii 6-fluoromevalonate 5'-phosphate