EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.35 | expression in Escherichia coli | Metallosphaera sedula |
2.3.1.9 | overexpressed as soluble N-terminal His10-tagged protein in Escherichia coli | Pyrobaculum neutrophilum |
4.2.1.150 | expression in Escherichia coli | Metallosphaera sedula |
6.2.1.40 | expression in Escherichia coli. The recombinant enzyme is modified by acetylation at Lys594 and is partly inactive | Pyrobaculum neutrophilum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.35 | 0.2 | - |
(S)-3-hydroxybutanoyl-CoA | pH 8.0, 65°C | Metallosphaera sedula | |
1.1.1.35 | 0.25 | - |
NAD+ | pH 8.0, 65°C | Metallosphaera sedula | |
2.3.1.9 | 0.15 | - |
acetoacetyl-CoA | pH 7.8, 85°C | Pyrobaculum neutrophilum | |
4.2.1.150 | 0.3 | - |
crotonyl-CoA | pH 8.0, 65°C | Metallosphaera sedula | |
6.2.1.40 | 0.7 | - |
4-hydroxybutanoate | pH 7.8, 85°C | Pyrobaculum neutrophilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.35 | 70000 | - |
x * 70000, SDS-PAGE | Metallosphaera sedula |
2.3.1.9 | 42444 | - |
x * 42444, calculated from sequence | Pyrobaculum neutrophilum |
4.2.1.150 | 70000 | - |
x * 70000, SDS-PAGE | Metallosphaera sedula |
6.2.1.40 | 71356 | - |
x * 71356, calculated from sequence | Pyrobaculum neutrophilum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.35 | (S)-3-hydroxybutanoyl-CoA + NAD+ | Metallosphaera sedula | the enzyme is involved in autotrophic carbon fixation | acetoacetyl-CoA + NADH + H+ | - |
? | |
1.1.1.35 | (S)-3-hydroxybutanoyl-CoA + NAD+ | Metallosphaera sedula DSM 5348 | the enzyme is involved in autotrophic carbon fixation | acetoacetyl-CoA + NADH + H+ | - |
? | |
2.3.1.9 | CoA + acetoacetyl-CoA | Pyrobaculum neutrophilum | the enzyme is involved in autotrophic carbon fixation | 2 acetyl-CoA | - |
? | |
2.3.1.9 | CoA + acetoacetyl-CoA | Pyrobaculum neutrophilum DSM 2338 | the enzyme is involved in autotrophic carbon fixation | 2 acetyl-CoA | - |
? | |
4.2.1.150 | crotonyl-CoA + H2O | Metallosphaera sedula | the enzyme is involved in autotrophic carbon fixation | (S)-3-hydroxybutanoyl-CoA | - |
? | |
4.2.1.150 | crotonyl-CoA + H2O | Metallosphaera sedula DSM 5348 | the enzyme is involved in autotrophic carbon fixation | (S)-3-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | Pyrobaculum neutrophilum | the enzyme is involved in autotrophic carbon fixation | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | Pyrobaculum neutrophilum DSM 2338 | the enzyme is involved in autotrophic carbon fixation | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.35 | Metallosphaera sedula | A4YDS4 | - |
- |
1.1.1.35 | Metallosphaera sedula DSM 5348 | A4YDS4 | - |
- |
2.3.1.9 | Pyrobaculum neutrophilum | B1YB71 | - |
- |
2.3.1.9 | Pyrobaculum neutrophilum DSM 2338 | B1YB71 | - |
- |
4.2.1.150 | Metallosphaera sedula | A4YDS4 | - |
- |
4.2.1.150 | Metallosphaera sedula DSM 5348 | A4YDS4 | - |
- |
6.2.1.40 | Pyrobaculum neutrophilum | B1YBY4 | - |
- |
6.2.1.40 | Pyrobaculum neutrophilum DSM 2338 | B1YBY4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.35 | - |
Metallosphaera sedula |
2.3.1.9 | - |
Pyrobaculum neutrophilum |
4.2.1.150 | - |
Metallosphaera sedula |
6.2.1.40 | - |
Pyrobaculum neutrophilum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.35 | culture condition:autotrophically-grown cell | bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase | Metallosphaera sedula | - |
1.1.1.35 | culture condition:heterotrophically-grown cell | bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase | Metallosphaera sedula | - |
4.2.1.150 | culture condition:autotrophically-grown cell | - |
Metallosphaera sedula | - |
4.2.1.150 | culture condition:heterotrophically-grown cell | - |
Metallosphaera sedula | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.35 | 3 | 8 | pH 8.0, 65°C, native enzyme, autotrophically- or heterotrophically-grown cell | Metallosphaera sedula |
2.3.1.9 | 55 | - |
pH 7.2, 85°C | Pyrobaculum neutrophilum |
4.2.1.150 | 3 | 8 | pH 8.0, 65°C, native enzyme, autotrophically- or heterotrophically-grown cell | Metallosphaera sedula |
6.2.1.40 | 1.6 | - |
pH 7.8, 85°C | Pyrobaculum neutrophilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.35 | (S)-3-hydroxybutanoyl-CoA + NAD+ | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula | acetoacetyl-CoA + NADH + H+ | - |
? | |
1.1.1.35 | (S)-3-hydroxybutanoyl-CoA + NAD+ | no activity with (R)-3-hydroxybutanoyl-CoA, no activity with NADP+. Bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula | acetoacetyl-CoA + NADH + H+ | - |
? | |
1.1.1.35 | (S)-3-hydroxybutanoyl-CoA + NAD+ | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula DSM 5348 | acetoacetyl-CoA + NADH + H+ | - |
? | |
1.1.1.35 | (S)-3-hydroxybutanoyl-CoA + NAD+ | no activity with (R)-3-hydroxybutanoyl-CoA, no activity with NADP+. Bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula DSM 5348 | acetoacetyl-CoA + NADH + H+ | - |
? | |
2.3.1.9 | CoA + acetoacetyl-CoA | - |
Pyrobaculum neutrophilum | 2 acetyl-CoA | - |
? | |
2.3.1.9 | CoA + acetoacetyl-CoA | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum | 2 acetyl-CoA | - |
? | |
2.3.1.9 | CoA + acetoacetyl-CoA | - |
Pyrobaculum neutrophilum DSM 2338 | 2 acetyl-CoA | - |
? | |
2.3.1.9 | CoA + acetoacetyl-CoA | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum DSM 2338 | 2 acetyl-CoA | - |
? | |
4.2.1.150 | crotonyl-CoA + H2O | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula | (S)-3-hydroxybutanoyl-CoA | - |
? | |
4.2.1.150 | crotonyl-CoA + H2O | bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula | (S)-3-hydroxybutanoyl-CoA | - |
? | |
4.2.1.150 | crotonyl-CoA + H2O | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula DSM 5348 | (S)-3-hydroxybutanoyl-CoA | - |
? | |
4.2.1.150 | crotonyl-CoA + H2O | bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula DSM 5348 | (S)-3-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 3-hydroxybutyrate + CoA | 8% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum | AMP + diphosphate + 3-hydroxybutyryl-CoA | - |
? | |
6.2.1.40 | ATP + 3-hydroxybutyrate + CoA | 8% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + 3-hydroxybutyryl-CoA | - |
? | |
6.2.1.40 | ATP + 3-hydroxypropionate + CoA | 9% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum | AMP + diphosphate + 3-hydroxypropionyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | - |
Pyrobaculum neutrophilum | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | - |
Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + 4-hydroxybutanoate + CoA | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + 4-hydroxybutanoyl-CoA | - |
? | |
6.2.1.40 | ATP + acetate + CoA | 13% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum | AMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.40 | ATP + acetate + CoA | 13% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + acetyl-CoA | - |
? | |
6.2.1.40 | ATP + crotonate + CoA | 22% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum | AMP + diphosphate + crotonyl-CoA | - |
? | |
6.2.1.40 | ATP + crotonate + CoA | 22% of the activity compared to 4-hydroxybutanoate | Pyrobaculum neutrophilum DSM 2338 | AMP + diphosphate + crotonyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.35 | ? | x * 70000, SDS-PAGE | Metallosphaera sedula |
2.3.1.9 | ? | x * 42444, calculated from sequence | Pyrobaculum neutrophilum |
4.2.1.150 | ? | x * 70000, SDS-PAGE | Metallosphaera sedula |
6.2.1.40 | ? | x * 71356, calculated from sequence | Pyrobaculum neutrophilum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.35 | Msed_0399 | locus name, bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula |
2.3.1.9 | beta-ketothiolase | - |
Pyrobaculum neutrophilum |
2.3.1.9 | Tneu_0249 | locus name | Pyrobaculum neutrophilum |
4.2.1.150 | Msed_0399 | locus name, bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula |
6.2.1.40 | Tneu_0420 | locus name | Pyrobaculum neutrophilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.35 | 65 | - |
assay at | Metallosphaera sedula |
4.2.1.150 | 65 | - |
assay at | Metallosphaera sedula |
6.2.1.40 | 85 | - |
assay at | Pyrobaculum neutrophilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.35 | 8 | - |
assay at | Metallosphaera sedula |
4.2.1.150 | 8 | - |
assay at | Metallosphaera sedula |
6.2.1.40 | 7.8 | - |
assay at | Pyrobaculum neutrophilum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.35 | NAD+ | no activity with NADP+ | Metallosphaera sedula |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.35 | physiological function | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula |
2.3.1.9 | physiological function | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum |
4.2.1.150 | physiological function | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula |
6.2.1.40 | physiological function | the enzyme is involved in autotrophic carbon fixation | Pyrobaculum neutrophilum |