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Literature summary extracted from

  • Janoir, C.; Pechine, S.; Grosdidier, C.; Collignon, A.
    Cwp84, a surface-associated protein of Clostridium difficile, is a cysteine protease with degrading activity on extracellular matrix proteins (2007), J. Bacteriol., 189, 7174-7180.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.15 dithiothreitol proteolytic activity requires presence of dithiothreitol. Autoproteolytic maturation of 68000 Da precursor to 61000 Da mature enzyme in presence of dithiothreitol Clostridioides difficile

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.15 expression in Escherichia coli Clostridioides difficile

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 E64 0.1 mM, complete inhibition Clostridioides difficile
3.4.22.15 EDTA 5 mM, 81% residual activity Clostridioides difficile
3.4.22.15 iodoacetic acid 10 mM, 15% residual activity Clostridioides difficile
3.4.22.15 leupeptin 0.1 mM, less than 10% residual activity Clostridioides difficile
3.4.22.15 N-ethylmaleimide 10 mM, less than 10% residual activity Clostridioides difficile
3.4.22.15 pepstatin 0.01 mM, 90% residual activity Clostridioides difficile
3.4.22.15 phenylmethylsulfonyl fluoride 10 mM, less than 10% residual activity Clostridioides difficile
3.4.22.15 TLCK 10 mM, less than 10% residual activity Clostridioides difficile

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.15 additional information enzyme is found to be associated with the S-layer proteins, mostly as a nonmature species Clostridioides difficile
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 61000
-
x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE Clostridioides difficile
3.4.22.15 68000
-
x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE Clostridioides difficile

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Clostridioides difficile Q83UF3
-
-
3.4.22.15 Clostridioides difficile 79-685 Q83UF3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.15 proteolytic modification autoproteolytic maturation of 68000 Da precursor to 61000 Da mature enzyme in presence of dithiothreitol or trypsin Clostridioides difficile

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15
-
Clostridioides difficile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 fibronectin + H2
-
Clostridioides difficile ? enzyme cleaves fibronectin to produce two bands ?
3.4.22.15 fibronectin + H2
-
Clostridioides difficile 79-685 ? enzyme cleaves fibronectin to produce two bands ?
3.4.22.15 laminin + H2
-
Clostridioides difficile ?
-
?
3.4.22.15 laminin + H2
-
Clostridioides difficile 79-685 ?
-
?
3.4.22.15 additional information proteolytic activity only under reducing conditions, e.g. in presence of dithiothreitol. No substrate: type IV collagen Clostridioides difficile ?
-
?
3.4.22.15 additional information proteolytic activity only under reducing conditions, e.g. in presence of dithiothreitol. No substrate: type IV collagen Clostridioides difficile 79-685 ?
-
?
3.4.22.15 vitronectin + H2
-
Clostridioides difficile ?
-
?
3.4.22.15 vitronectin + H2
-
Clostridioides difficile 79-685 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ? x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE Clostridioides difficile

Synonyms

EC Number Synonyms Comment Organism
3.4.22.15 Cwp84
-
Clostridioides difficile

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 7.5
-
-
Clostridioides difficile

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.15 3.5 8 more than 80% of maximum activity Clostridioides difficile
3.4.22.15 10
-
30% of maximum activity Clostridioides difficile

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.22.15 Clostridioides difficile isoelectric focusing
-
7.5