EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.15 | dithiothreitol | proteolytic activity requires presence of dithiothreitol. Autoproteolytic maturation of 68000 Da precursor to 61000 Da mature enzyme in presence of dithiothreitol | Clostridioides difficile |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.15 | expression in Escherichia coli | Clostridioides difficile |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.15 | E64 | 0.1 mM, complete inhibition | Clostridioides difficile | |
3.4.22.15 | EDTA | 5 mM, 81% residual activity | Clostridioides difficile | |
3.4.22.15 | iodoacetic acid | 10 mM, 15% residual activity | Clostridioides difficile | |
3.4.22.15 | leupeptin | 0.1 mM, less than 10% residual activity | Clostridioides difficile | |
3.4.22.15 | N-ethylmaleimide | 10 mM, less than 10% residual activity | Clostridioides difficile | |
3.4.22.15 | pepstatin | 0.01 mM, 90% residual activity | Clostridioides difficile | |
3.4.22.15 | phenylmethylsulfonyl fluoride | 10 mM, less than 10% residual activity | Clostridioides difficile | |
3.4.22.15 | TLCK | 10 mM, less than 10% residual activity | Clostridioides difficile |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.22.15 | additional information | enzyme is found to be associated with the S-layer proteins, mostly as a nonmature species | Clostridioides difficile | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.15 | 61000 | - |
x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE | Clostridioides difficile |
3.4.22.15 | 68000 | - |
x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE | Clostridioides difficile |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.15 | Clostridioides difficile | Q83UF3 | - |
- |
3.4.22.15 | Clostridioides difficile 79-685 | Q83UF3 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.22.15 | proteolytic modification | autoproteolytic maturation of 68000 Da precursor to 61000 Da mature enzyme in presence of dithiothreitol or trypsin | Clostridioides difficile |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.15 | - |
Clostridioides difficile |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.15 | fibronectin + H2 | - |
Clostridioides difficile | ? | enzyme cleaves fibronectin to produce two bands | ? | |
3.4.22.15 | fibronectin + H2 | - |
Clostridioides difficile 79-685 | ? | enzyme cleaves fibronectin to produce two bands | ? | |
3.4.22.15 | laminin + H2 | - |
Clostridioides difficile | ? | - |
? | |
3.4.22.15 | laminin + H2 | - |
Clostridioides difficile 79-685 | ? | - |
? | |
3.4.22.15 | additional information | proteolytic activity only under reducing conditions, e.g. in presence of dithiothreitol. No substrate: type IV collagen | Clostridioides difficile | ? | - |
? | |
3.4.22.15 | additional information | proteolytic activity only under reducing conditions, e.g. in presence of dithiothreitol. No substrate: type IV collagen | Clostridioides difficile 79-685 | ? | - |
? | |
3.4.22.15 | vitronectin + H2 | - |
Clostridioides difficile | ? | - |
? | |
3.4.22.15 | vitronectin + H2 | - |
Clostridioides difficile 79-685 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.15 | ? | x * 68000, precursor, x * 61000, mature proetin, SDS-PAGE | Clostridioides difficile |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.15 | Cwp84 | - |
Clostridioides difficile |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.15 | 7.5 | - |
- |
Clostridioides difficile |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.15 | 3.5 | 8 | more than 80% of maximum activity | Clostridioides difficile |
3.4.22.15 | 10 | - |
30% of maximum activity | Clostridioides difficile |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.22.15 | Clostridioides difficile | isoelectric focusing | - |
7.5 |