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Literature summary extracted from

  • Kordel, M.; Hofmann, B.; Schomburg, D.; Schmid, R.D.
    Extracellular lipase of Pseudomonas sp. strain ATCC 21808: purification, characterization, crystallization, and preliminary X-ray diffraction data (1991), J. Bacteriol., 173, 4836-4841.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.3 to establish the best crystallization conditions, the hanging-drop method was used (room temperature). Crystals suitable for X-ray diffraction grow in sitting drops. The lipase crystallizes with different salts and ethylene glycol polymers in the presence of n-octyl-beta-D-glucopyranoside and one alkyloligooxyethylene compound in the range from C5E2 to C8E4. The crystals diffract to a resolution of about 0.25 nm. They belong to space group C2 with lattice constants of a = 9.27 nm, b = 4.74 nm, c = 8.65 nm, and beta = 122.3°, indicating a cell content of one molecule per asymmetric unit of the crystal Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 additional information no inhibition with PMSF Burkholderia cepacia
3.1.1.3 paraoxon inhibition of the lipase activities toward emulsified triolein and dissolved p-nitrophenyl acetate by a 1000fold molar excess of paraoxon, 1 h, 99% inhibition Burkholderia cepacia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Burkholderia cepacia
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 35000
-
 x * 35000, SDS-PAGE Burkholderia cepacia
3.1.1.3 110000
-
 polyacrylamide gel under nondenaturing conditions Burkholderia cepacia

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 2-propanol 22°C, stable to Burkholderia cepacia
3.1.1.3 Ethanol 22°C, crude enzyme is significantly more resistant to higher concentrations of ethanol (above 40%) than to lower concentrations (20%), whereas purified lipase is inactivated by concentrations of above 40% Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Burkholderia cepacia P22088 i.e. Pseudomonas cepacia
-
3.1.1.3 Burkholderia cepacia ATCC 21808 P22088 i.e. Pseudomonas cepacia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 chromatography on Q-Sepharose in the presence of n-octyl-beta-D-glucopyranoside, Ca2+ precipitation of fatty acids, and octyl-Sepharose chromatography Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information substrate specificity for saturated fatty acids from C6 to C12 and unsaturated long-chain fatty acids. Monoglycerides are hydrolyzed very slowly Burkholderia cepacia ?
-
 ?
3.1.1.3 additional information substrate specificity for saturated fatty acids from C6 to C12 and unsaturated long-chain fatty acids. Monoglycerides are hydrolyzed very slowly Burkholderia cepacia ATCC 21808 ?
-
 ?
3.1.1.3 triolein + H2O
-
 Burkholderia cepacia diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Burkholderia cepacia ATCC 21808 diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 35000, SDS-PAGE Burkholderia cepacia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 assay at Burkholderia cepacia

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Burkholderia cepacia isoelectric focusing 4.6 4.5