EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.2 | gene oxdC, cloning and expression in Escherichia coli strain DH5alpha and BL21(DE3), constitutive overexpression from plasmid pLB36, with promoter PldhL from Lactobacillus plantarum NCIMB8826, in Lactobacillus plantarum strain NC8. The recombinant Lactobacillus plantarum is able to degrade more than 90% of added oxalate, compared to 15% by the wild-type, and also shows higher tolerance to oxalate | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.2 | Mn2+ | required | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.2 | oxalate + H+ | Bacillus subtilis | - |
formate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.2 | Bacillus subtilis | - |
gene oxdC | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.2 | additional information | - |
specific activity of oxalate decarboxylase in crude cell-free extract of recombinant Lactobacillus plantarum NC8 and Escherichia coli (pLB36) compared with wild-type Lactobacillus plantarum NC8 | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.2 | oxalate + H+ | - |
Bacillus subtilis | formate + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.2 | OXDC | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.2 | 25 | - |
assay at | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.2 | 4 | - |
assay at | Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.2 | additional information | - |
inactive at pH 7.5 | Bacillus subtilis |