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Literature summary extracted from

  • Anbazhagan, K.; Sasikumar, P.; Gomathi, S.; Priya, H.P.; Selvam, G.S.
    In vitro degradation of oxalate by recombinant Lactobacillus plantarum expressing heterologous oxalate decarboxylase (2013), J. Appl. Microbiol., 115, 880-887.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.2 gene oxdC, cloning and expression in Escherichia coli strain DH5alpha and BL21(DE3), constitutive overexpression from plasmid pLB36, with promoter PldhL from Lactobacillus plantarum NCIMB8826, in Lactobacillus plantarum strain NC8. The recombinant Lactobacillus plantarum is able to degrade more than 90% of added oxalate, compared to 15% by the wild-type, and also shows higher tolerance to oxalate Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.2 Mn2+ required Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.2 oxalate + H+ Bacillus subtilis
-
formate + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.2 Bacillus subtilis
-
gene oxdC
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.2 additional information
-
specific activity of oxalate decarboxylase in crude cell-free extract of recombinant Lactobacillus plantarum NC8 and Escherichia coli (pLB36) compared with wild-type Lactobacillus plantarum NC8 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.2 oxalate + H+
-
Bacillus subtilis formate + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.2 OXDC
-
Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.2 25
-
assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.2 4
-
assay at Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.2 additional information
-
inactive at pH 7.5 Bacillus subtilis