EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.6 | gene cadA, heterologous functional expression in citric acid producing Aspergillus niger under various constitutive promoters of different expression strength, heterologous expression of the gene in Saccharomyces cerevisiae | Aspergillus terreus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.6 | additional information | a truncated version of the Aspergillus niger pfkA gene exhibits a higher citric acid yield due to a reduced inhibition by citrate and ATP, the truncated pfkA version has also a positive impact on the itaconic acid accumulation when expressed in Aspergillus terreus | Aspergillus terreus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.6 | 2.45 | - |
cis-aconitate | pH 6.2, 37°C | Aspergillus terreus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.6 | cytosol | - |
Aspergillus terreus | 5829 | - |
4.1.1.6 | cytosol | - |
Aspergillus itaconicus | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.6 | 55000 | - |
x * 55000, recombinant enzyme, SDS-PAGE | Aspergillus terreus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.6 | cis-aconitate | Aspergillus terreus | - |
itaconate + CO2 | - |
? | |
4.1.1.6 | cis-aconitate | Aspergillus itaconicus | - |
itaconate + CO2 | - |
? | |
4.1.1.6 | cis-aconitate | Aspergillus terreus NRRL1960 | - |
itaconate + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.6 | Aspergillus itaconicus | - |
gene cadA | - |
4.1.1.6 | Aspergillus terreus | - |
gene cadA | - |
4.1.1.6 | Aspergillus terreus NRRL1960 | - |
gene cadA | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.1.6 | additional information | production of itaconic acid is achieved by the fermentation with Aspergillus terreus on sugar-containing media | Aspergillus terreus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.6 | cis-aconitate | - |
Aspergillus terreus | itaconate + CO2 | - |
? | |
4.1.1.6 | cis-aconitate | - |
Aspergillus itaconicus | itaconate + CO2 | - |
? | |
4.1.1.6 | cis-aconitate | - |
Aspergillus terreus NRRL1960 | itaconate + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.6 | ? | x * 55000, recombinant enzyme, SDS-PAGE | Aspergillus terreus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.6 | CadA | - |
Aspergillus terreus |
4.1.1.6 | CadA | - |
Aspergillus itaconicus |
4.1.1.6 | cis-aconitate decarboxylase | - |
Aspergillus terreus |
4.1.1.6 | cis-aconitate decarboxylase | - |
Aspergillus itaconicus |
4.1.1.6 | cis-Aconitic acid decarboxylase | - |
Aspergillus terreus |
4.1.1.6 | cis-Aconitic acid decarboxylase | - |
Aspergillus itaconicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.6 | 37 | - |
- |
Aspergillus terreus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.6 | 6.2 | - |
- |
Aspergillus terreus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.1.6 | evolution | the enzyme belongs to the MmgE/PrpD family | Aspergillus terreus |
4.1.1.6 | metabolism | in the first steps of the citric acid cycle, citrate and cis-aconitate are formed, the enzymes preceding in the pathway, namely citrate synthase and aconitase, are found in the mitochondria. In the last step, the only itaconic acid pathway dedicated step, cis-aconitate decarboxylase (CadA) forms itaconic acid releasing carbon dioxide in the cytosol | Aspergillus terreus |
4.1.1.6 | metabolism | in the first steps of the citric acid cycle, citrate and cis-aconitate are formed, the enzymes preceding in the pathway, namely citrate synthase and aconitase, are found in the mitochondria. In the last step, the only itaconic acid pathway dedicated step, cis-aconitate decarboxylase (CadA) forms itaconic acid releasing carbon dioxide in the cytosol | Aspergillus itaconicus |